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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using NSD3 Recombinant antibody (83116-2-RR)

    WB analysis of A549 using 83116-2-RR (same clone as 83116-2-PBS)

    WB result of NSD3 antibody (83116-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSD3 transfected A549 cells. This data was developed using the same antibody clone with 83116-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NSD3 Recombinant antibody (83116-2-RR)

    WB analysis using 83116-2-RR (same clone as 83116-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83116-2-RR (WHSC1L1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83116-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00189-4

    Sandwich ELISA standard curve of MP00189-4

    Sandwich ELISA standard curve of MP00189-4, Human NSD3 Recombinant Matched Antibody Pair - PBS only. 83116-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1894. 83116-5-PBS was HRP conjugated as the detection antibody. Range: 19.5-625 pg/mL.

  • Sample test of MP00189-4

    Sample test of MP00189-4

    The mean NSD3 concentration was determined to be 542.5 pg/mL in A549 cell extract based on a 1.0 mg/mL extract load, 565.1 pg/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load and 529.6 pg/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

  • Immunohistochemistry (IHC) staining of human lung squamous cell carcinoma tissue using NSD3 Recombinant antibody (83116-2-RR)

    IHC staining of human lung squamous cell carcinoma using 83116-2-RR (same clone as 83116-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 83116-2-RR (NSD3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83116-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using NSD3 Recombinant antibody (83116-2-RR)

    IF Staining of A549 using 83116-2-RR (same clone as 83116-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using NSD3 antibody (83116-2-RR, Clone: 230499C2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83116-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using NSD3 Recombinant antibody (83116-2-RR)

    FC experiment of HeLa using 83116-2-RR (same clone as 83116-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug NSD3 Recombinant antibody (83116-2-RR, Clone:230499C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83116-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83116-2-RR

    Affinity and Kinetic Characterization of 83116-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83116-2-RR against Human NSD3 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

NSD3 Recombinant monoclonal antibody, PBS Only (Capture)

NSD3 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83116-2-PBS
Clone No.230499C2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

WHSC1L1, DC28, EC:2.1.1.370, Histone-lysine N-methyltransferase NSD3, Nuclear SET domain-containing protein 3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83116-2-PBS targets NSD3 as part of a matched antibody pair:

MP00189-4: 83116-2-PBS capture and 83116-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1894

Product name: Recombinant human WHSC1L1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC012059

Sequence: MDFSFSFMQGIMGNTIQQPPQLIDSANIRQEDAFDNNSDIAEDGGQTPYEATLQQGFQYPATTEDLPPLTNGYPSSISVYETQTKYQSYNQYPNGSANGFGAVRNFSPTDYYHSEIPNTRPHEILEKPSPPQPPPPPSVPQTVIPKKTGSPEIKLKITKTIQNGRELFESSLCGDLLNEVQASEHTKSKHESRKEKRKKSNKHDSSRSEERKSHKIPKLEPEEQNRPNERVDTVSEKPREEPVLKEEAPVQPILSSVPTTEVSTGVKFQVGDLVWSKVGTYPWWPCMVSSDPQLEVHTKI

相同性解析による交差性が予測される生物種
Full Name Wolf-Hirschhorn syndrome candidate 1-like 1
Calculated molecular weight 1437 aa, 162 kDa
Observed molecular weight 160-170 kDa, 68-72 kDa
GenBank accession numberBC012059
Gene Symbol WHSC1L1
Gene ID (NCBI) 54904
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9BZ95
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

WHSC1L1, also named as NSD3 and DC28, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is histone methyltransferase. WHSC1L1 is a potently transforming oncogenes based on the number of altered phenotypes expressed by the cells. It contains a PWWP-domain that is a methyl-lysine recognition motif involved in histone code modification and epigenetic regulation of gene expression. Knockdown of WHSC1L1 in 8p11-12-amplified breast cancer cells resulted in profound loss of growth and survival of these cells. (PMID: 20940404). This antibody recognize the long isoform (160-170 kDa) and short isoform (68-72 kDa).

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