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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WHAMM Polyclonal antibody (31572-1-AP)

    WB analysis using 31572-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31572-1-AP (WHAMM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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WHAMM Polyclonal antibody

WHAMM Polyclonal Antibody for WB, ELISA
Cat No. 31572-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

WHDC1, KIAA1971

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inDaudi cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31572-1-AP targets WHAMM in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WHAMM fusion protein Ag36160 相同性解析による交差性が予測される生物種
Full Name WAS protein homolog associated with actin, golgi membranes and microtubules
Observed molecular weight90-100 kDa
GenBank accession numberBC167774
Gene Symbol WHAMM
Gene ID (NCBI) 123720
RRIDAB_3670039
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8TF30
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WHAMM (WASP homolog associated with actin, golgi membranes and microtubules), also known as WHDC1. It is expected to be located in the cytoplasm, which is enriched in brain, lung, heart, colon and kidney. WHAMM is involved in Golgi membrane association, microtubule binding, and actin nucleation as a nucleation-promoting factor, which activates the actin-related protein 2/3 complex (the Arp2/ 3 complex) (PMID: 23160625). It is reported that the nucleation-promoting factor (NPF) WHAMM recruits and activates the Arp2/3 complex for actin assembly at sites of autophagosome formation on the ER (PMID: 26291929). The molecular weight of WHAMM is 90 kDa.

Protocols

Product Specific Protocols
WB protocol for WHAMM antibody 31572-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols