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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using WEE2 Polyclonal antibody (55119-1-AP)

    WB analysis of Jurkat using 55119-1-AP (same clone as 55119-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55119-1-AP (WEE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55119-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human ovary tissue using WEE2 Polyclonal antibody (55119-1-AP)

    WB analysis of human ovary using 55119-1-AP (same clone as 55119-1-PBS)

    human ovary tissue were subjected to SDS PAGE followed by western blot with 55119-1-AP (WEE2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55119-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using WEE2 Polyclonal antibody (55119-1-AP)

    WB analysis of Jurkat using 55119-1-AP (same clone as 55119-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55119-1-AP (WEE2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55119-1-PBS in a different storage buffer formulation.

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WEE2 Polyclonal antibody, PBS Only

WEE2 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 55119-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55119-1-PBS targets WEE2 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name WEE1 homolog 2 (S. pombe)
Calculated molecular weight 63 kDa
Observed molecular weight 63-66 kDa
GenBank accession numberNM_001105558
Gene Symbol WEE2
Gene ID (NCBI) 494551
RRIDAB_10951857
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP0C1S8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

WEE2, also named as WEE1B, belongs to the protein kinase superfamily. Ser/Thr protein kinase family and WEE1 subfamily. It phosphorylates and inhibits CDK1. WEE2 may act as a negative regulator of entry into mitosis (G2 to M transition). WEE2 is conserved from yeast to humans. The aim of the present study was to determine the spatiotemporal expression pattern and the function of WEE2 during oocyte maturation in a nonhuman primate species, the rhesus macaque. Taking the long double-stranded RNA-mediated RNA interference approach, down-regulation of WEE2 led to meiotic resumption in a subset of GV oocytes even in the presence of a phosphodiesterase 3 inhibitor. Overexpression of WEE2 delays the reentry of oocytes into meiosis in both mice and monkeys. WEE2 is a conserved oocyte-specific meiosis inhibitor that functions downstream of cAMP in nonhuman primates.(PMID: 20200212) The antibody is specific to WEE2.