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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WDR92 Polyclonal antibody (25540-1-AP)

    WB analysis using 25540-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25540-1-AP (WDR92 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using WDR92 Polyclonal antibody (25540-1-AP)

    IHC staining of human colon using 25540-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25540-1-AP (WDR92 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using WDR92 Polyclonal antibody (25540-1-AP)

    IHC staining of human testis using 25540-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25540-1-AP (WDR92 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using WDR92 Polyclonal antibody (25540-1-AP)

    IHC staining of human colon using 25540-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 25540-1-AP (WDR92 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using WDR92 Polyclonal antibody (25540-1-AP)

    IHC staining of mouse testis using 25540-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25540-1-AP (WDR92 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using WDR92 Polyclonal antibody (25540-1-AP)

    IHC staining of mouse testis using 25540-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25540-1-AP (WDR92 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using WDR92 Polyclonal antibody (25540-1-AP)

    IHC staining of human colon using 25540-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25540-1-AP (WDR92 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using WDR92 Polyclonal antibody (25540-1-AP)

    IHC staining of human testis using 25540-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25540-1-AP (WDR92 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using WDR92 Polyclonal antibody (25540-1-AP)

    IF Staining of HepG2 using 25540-1-AP

    Immunofluorescent analysis of HepG2 cells using 25540-1-AP (WDR92 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

WDR92 Polyclonal antibody

WDR92 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25540-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

WD repeat-containing protein Monad, WD repeat-containing protein 92, WD repeat domain 92, Dynein axonemal assembly factor 10, DNAAF10

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells
Positive IHC detected inhuman colon tissue, human testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25540-1-AP targets WDR92 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22181

Product name: Recombinant human WDR92 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-357 aa of BC066657

Sequence: MSAFEKPQIIAHIQKGFNYTVFDCKWVPCSAKFVTMGNFARGTGVIQLYEIQHGDLKLLREIEKAKPIKCGTFGATSLQQRYLATGDFGGNLHIWNLEAPEMPVYSVKGHKEIINAIDGIGGLGIGEGAPEIVTGSRDGTVKVWDPRQKDDPVANMEPVQGENKRDCWTVAFGNAYNQEERVVCAGYDNGDIKLFDLRNMALRWETNIKNGVCSLEFDRKDISMNKLVATSLEGKFHVFDMRTQHPTKGFASVSEKAHKSTVWQVRHLPQNRELFLTAGGAGGLHLWKYEYPIQRSKKDSEGIEMGVAGSVSLLQNVTLSTQPISSLDWSPDKRGLCVCSSFDQTVRVLIVTKLNKI

相同性解析による交差性が予測される生物種
Full Name WD repeat domain 92
Calculated molecular weight 357 aa, 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC066657
Gene Symbol WDR92
Gene ID (NCBI) 116143
RRIDAB_2880124
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96MX6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for WDR92 antibody 25540-1-APDownload protocol
IHC protocol for WDR92 antibody 25540-1-APDownload protocol
WB protocol for WDR92 antibody 25540-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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