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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WDR81 Polyclonal antibody (24874-1-AP)

    WB analysis using 24874-1-AP (same clone as 24874-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24874-1-AP (WDR81 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24874-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using WDR81 Polyclonal antibody (24874-1-AP)

    WB analysis using 24874-1-AP (same clone as 24874-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24874-1-AP (WDR81 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24874-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using WDR81 Polyclonal antibody (24874-1-AP)

    IHC staining of mouse cerebellum using 24874-1-AP (same clone as 24874-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24874-1-AP (WDR81 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24874-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using WDR81 Polyclonal antibody (24874-1-AP)

    IHC staining of mouse cerebellum using 24874-1-AP (same clone as 24874-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24874-1-AP (WDR81 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24874-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using WDR81 Polyclonal antibody (24874-1-AP)

    IF Staining of mouse cerebellum using 24874-1-AP (same clone as 24874-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using WDR81 antibody (24874-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24874-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using WDR81 Polyclonal antibody (24874-1-AP)

    IF Staining of HepG2 using 24874-1-AP (same clone as 24874-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using WDR81 antibody (24874-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 24874-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

WDR81 Polyclonal antibody, PBS Only

WDR81 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 24874-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

WD repeat-containing protein 81

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24874-1-PBS targets WDR81 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21666

Product name: Recombinant human WDR81 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 323-692 aa of BC114568

Sequence: NDSRPENPGPLGPISGVGGGGLGSGSDDNALKQELPRSVHGLSGNWLAYWQYEIGVSQQDAHFHFHQIRLQSFPGHSGAVKCVAPLSSEDFFLSGSKDRTVRLWPLYNYGDGTSETAPRLVYTQHRKSVFFVGQLEAPQHVVSCDGAVHVWDPFTGKTLRTVEPLDSRVPLTAVAVMPAPHTSITMASSDSTLRFVDCRKPGLQHEFRLGGGLNPGLVRALAISPSGRSVVAGFSSGFMVLLDTRTGLVLRGWPAHEGDILQIKAVEGSVLVSSSSDHSLTVWKELEQKPTHHYKSASDPIHTFDLYGSEVVTGTVSNKIGVCSLLEPPSQATTKLSSENFRGTLTSLALLPTKRHLLLGSDNGVIRLLA

相同性解析による交差性が予測される生物種
Full Name WD repeat domain 81
Calculated molecular weight 1941 aa, 212 kDa
Observed molecular weight74-80 kDa, 250 kDa
GenBank accession numberBC114568
Gene Symbol WDR81
Gene ID (NCBI) 124997
RRIDAB_2879771
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ562E7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

WD repeat-containing protein 81 (WDR81) is a 1941 amino acid protein, which contains one BEACH domain and five WD repeats. WDR81 is widely expressed in the brain. WDR81 has an association with cerebellar ataxia, mental retardation, and dysequilibrium syndrome 2. WDR81 exists as six isoforms.

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