Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells, MG-63 cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33145-1-AP targets WDR70 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27019 Product name: Recombinant human WDR70 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 393-649 aa of BC009648 Sequence: LASRGGDDSLKLWDIRQFNKPLFSASGLPTMFPMTDCCFSPDDKLIVTGTSIQRGCGSGKLVFFERRTFQRVYEIDITDASVVRCLWHPKLNQIMVGTGNGLAKVYYDPNKSQRGAKLCVVKTQRKAKQAETLTQDYIITPHALPMFREPRQRSTRKQLEKDRLDPLKSHKPEPPVAGPGRGGRVGTHGGTLSSYIVKNIALDKTDDSNPREAILRHAKAAEDSPYWVSPAYSKTQPKTMFAQVESDDEEAKNEPEW 相同性解析による交差性が予測される生物種 |
| Full Name | WD repeat domain 70 |
| Calculated molecular weight | 654 aa, 73 kDa |
| Observed molecular weight | 70-75 kDa |
| GenBank accession number | BC009648 |
| Gene Symbol | WDR70 |
| Gene ID (NCBI) | 55100 |
| RRID | AB_3742870 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9NW82 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
WD repeat-containing protein 70 (WDR70), which is composed of six WD40 repeat domain functions as a subunit of CRL4 complex in homology-directed repair (HDR) by regulating the transcription of BRCA1, BRCA2, RAD51, and substantial additional transcriptomic changes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for WDR70 antibody 33145-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |