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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HEK-293T cells using WDR62 Polyclonal antibody (23404-1-AP)

    IP experiment of HEK-293T using 23404-1-AP

    IP result of anti-WDR62 (IP:23404-1-AP, 4ug; Detection:23404-1-AP 1:500) with HEK-293T cells lysate 1320 ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using WDR62 Polyclonal antibody (23404-1-AP)

    IHC staining of mouse testis using 23404-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23404-1-AP (WDR62 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using WDR62 Polyclonal antibody (23404-1-AP)

    IHC staining of mouse testis using 23404-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23404-1-AP (WDR62 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using WDR62 Polyclonal antibody (23404-1-AP)

    IF Staining of hTERT-RPE1 using 23404-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using WDR62 antibody (23404-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using WDR62 Polyclonal antibody (23404-1-AP)

    IF Staining of A549 using 23404-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using WDR62 antibody (23404-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

WDR62 Polyclonal antibody

WDR62 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA
Cat No. 23404-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, IP, ELISA

Synonyms

C19orf14, WD repeat domain 62, WD repeat-containing protein 62

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IP detected inHEK-293T cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells, A549 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23404-1-AP targets WDR62 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19865

Product name: Recombinant human WDR62 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 281-630 aa of BC017261

Sequence: CLTSLASCVPASSVLPTDRNLPTPTSAPTPGLAQGVHAPSTCSYMEATASSRARISRSISLGDSEGPIVATLAQPLRRPSSVGELASLGQELQAITTATTPSLDSEGQEPALRSWGNHEARANLRLTLSSACDGLLLPPVDTQPGVTVPAVSFPAPSPVEESALRLHGSAFRPSLPAPESPGLPAHPSNPQLPEARPGIPGSTASLLEPTSGALGLFQGSPARWSEPWVPVEALPPSPLELSRVGNILHRLQTTFQEALDLYRVLVSSGQVDTGQQQARTELVSTFLWIHSQLEAECLVGTSVAPAQALPSPGPPSPPTLYPLASPDLQALLEHYSELLVQAVRRKARGH

相同性解析による交差性が予測される生物種
Full Name WD repeat domain 62
Calculated molecular weight 1518 aa, 166 kDa
Observed molecular weight180 kDa
GenBank accession numberBC017261
Gene Symbol WDR62
Gene ID (NCBI) 284403
RRIDAB_3085711
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDO43379
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The WD40-repeat protein 62 (WDR62) is a scaffold protein that recruits different components of the JNK signaling pathway to regulate several human diseases including neurological disorders, infertility, and tumorigenesis (PMID: 33937237). Moreover, WDR62 participates in diverse biological processes, especially mitotic spindle organization via regulating centriole biogenesis and the function of centriole-associated protein (PMID: 35093571).

Protocols

Product Specific Protocols
IF protocol for WDR62 antibody 23404-1-APDownload protocol
IHC protocol for WDR62 antibody 23404-1-APDownload protocol
IP protocol for WDR62 antibody 23404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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