Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, mouse spleen tissue, rat spleen tissue |
| Positive IP detected in | Jurkat cells |
| Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 3 publications below |
Product Information
15110-1-AP targets WDR46 in WB, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | WDR46 fusion protein Ag7095 相同性解析による交差性が予測される生物種 |
| Full Name | WD repeat domain 46 |
| Calculated molecular weight | 68 kDa |
| Observed molecular weight | 70-75 kDa |
| GenBank accession number | BC000388 |
| Gene Symbol | WDR46 |
| Gene ID (NCBI) | 9277 |
| RRID | AB_2215408 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15213 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for WDR46 antibody 15110-1-AP | Download protocol |
| IP protocol for WDR46 antibody 15110-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Genes Dev Role of the small subunit processome in the maintenance of pluripotent stem cells. | ||
Biochem Cell Biol Dynamics of WD-repeat containing proteins in SSU processome components. | ||
Genes Cells Nucleolar scaffold protein, WDR46, determines the granular compartmental localization of nucleolin and DDX21. | ||
Biochem Biophys Res Commun Nuclear localization of DMP1 proteins suggests a role in intracellular signaling. |