Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | MDCK cells, hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26701-1-AP targets WDR35 in IF/ICC, ELISA applications and shows reactivity with human, canine samples.
| Tested Reactivity | human, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24965 Product name: Recombinant human WDR35 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 822-1170 aa of BC036659 Sequence: AECYYMLEDYEGLENLAISLPENHKLLPEIAQMFVRVGMCEQAVTTFLKCSQPKAAVDTCVHLNQWNKAVELAKNHSMKEIGSLLARYASHLLEKNKTLDAIELYRKANYFFDAAKLMFKIADEEAKKGSKPLRVKKLYVLSALLIEQYHEQMKNAQRGKVKGKSSEATSALAGLLEEEVLSTTDRFTDNAWRGAEAYHFFILAQRQLYEGCVDTALKTALHLKDYEDIIPPVEIYSLLALCACAGRAFGTCSKAFIKLKSLETLSSEQKQQYEDLALEIFTKHTSKDNRKPELDSLMEGGEGKLPTCVATGSPITEYQFWMCSVCKHGVLAQEISHYSFCPLCHSPVG 相同性解析による交差性が予測される生物種 |
| Full Name | WD repeat domain 35 |
| Calculated molecular weight | 1170 aa, 132 kDa |
| GenBank accession number | BC036659 |
| Gene Symbol | WDR35 |
| Gene ID (NCBI) | 57539 |
| RRID | AB_3085893 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P2L0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
WDR35, a component of the IFT complex A (IFT-A), is required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs). It is involved in ciliogenesis and ciliary protein trafficking (PMID:21473986, PMID:28400947, PMID:29220510). It may promote CASP3 activation and TNF-stimulated apoptosis (PMID: 20193664).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for WDR35 antibody 26701-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |