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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WDR21C Recombinant antibody (83802-4-RR)

    WB analysis using 83802-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83802-4-RR (WDR21C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83802-4-RR

    Affinity and Kinetic Characterization of 83802-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83802-4-RR against Human WDR21C were performed. The affinity constant is 0.479 nM.

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WDR21C Recombinant antibody

WDR21C Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83802-4-RR
Clone No.240402H3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

WD repeat-containing protein 21C, WD repeat domain 21C, DDB1- and CUL4-associated factor 4-like protein 2, DCAF4L2, 240402H3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, U-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83802-4-RR targets WDR21C in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen WDR21C fusion protein Ag30748 相同性解析による交差性が予測される生物種
Full Name WD repeat domain 21C
Calculated molecular weight 395 aa, 44 kDa
Observed molecular weight44-50 kDa
GenBank accession numberBC111778
Gene Symbol WDR21C
Gene ID (NCBI) 138009
RRIDAB_3671390
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NA75
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WDR21C (WD repeat-containing protein 21C), also known as DCAF4L2. It is expected to be located in the cytoplasm, which is enriched in testis. WDR21C is a member of WD-repeat proteins, which commonly serve as mediators of protein-protein interplay. It is reported that elevated WDR21C expression in human colorectal cancer (CRC) significantly correlated with a more advanced clinical stage as in lymphatic and distant metastasis. More importantly, elevated WDR21C expression is an independent prognosis factor for survival (PMID: 27158335). The molecular weight of WDR21C is 43 kDa.

Protocols

Product Specific Protocols
WB protocol for WDR21C antibody 83802-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols