Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human lung tissue, human lung cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IP | See 1 publications below |
Product Information
15165-1-AP targets WDR18 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7282 Product name: Recombinant human WDR18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-306 aa of BC001648 Sequence: MAAPMEVAVCTDSAAPMWSCIVWELHSGANLLTYRGGQAGPRGLALLNGEYLLAAQLGKNYISAWELQRKDQLQQKIMCPGPVTCLTASPNGLYVLAGVAESIHLWEVSTGNLLVILSRHYQDVSCLQFTGDSSHFISGGKDCLVLVWSLCSVLQADPSRIPAPRHVWSHHTLPITDLHCGFGGPLARVATSSLDQTVKLWEVSSGELLLSVLFDVSIMAVTMDLAEHHMFCGGSEGSIFQVDLFTWPGQRERSFHPEQDAGKVFKGHRNQVTCLSVSTDGSVLLSGSHDETVRLWDVQSKQCIRT 相同性解析による交差性が予測される生物種 |
| Full Name | WD repeat domain 18 |
| Calculated molecular weight | 47 kDa |
| Observed molecular weight | 47 kDa |
| GenBank accession number | BC001648 |
| Gene Symbol | WDR18 |
| Gene ID (NCBI) | 57418 |
| RRID | AB_2288469 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BV38 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
WDR18 is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WDR18 may play a role during development. The complex PELP1-TEX10-WDR18 is required for the maturation and nucleolar release of the large ribosomal subunit. This complex is a regulator of ribosome biogenesis and suggests that its SUMO-controlled distribution coordinates the rate of ribosome formation (PMID:21326211).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for WDR18 antibody 15165-1-AP | Download protocol |
| WB protocol for WDR18 antibody 15165-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J The SUMO system controls nucleolar partitioning of a novel mammalian ribosome biogenesis complex.
| ||
Mol Cell Proteomics Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation. | ||
Mol Oncol PELP1 inhibition by SMIP34 reduces endometrial cancer progression via attenuation of ribosomal biogenesis |
