Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33831-1-AP targets WDR12 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38016 Product name: Recombinant human WDR12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 74-423 aa of BC008082 Sequence: NISSEEVVEIEYVEKYTAPQPEQCMFHDDWISSIKGAEEWILTGSYDKTSRIWSLEGKSIMTIVGHTDVVKDVAWVKKDSLSCLLLSASMDQTILLWEWNVERNKVKALHCCRGHAGSVDSIAVDGSGTKFCSGSWDKMLKIWSTVPTDEEDEMEESTNRPRKKQKTEQLGLTRTPIVTLSGHMEAVSSVLWSDAEEICSASWDHTIRVWDVESGSLKSTLTGNKVFNCISYSPLCKRLASGSTDRHIRLWDPRTKDGSLVSLSLTSHTGWVTSVKWSPTHEQQLISGSLDNIVKLWDTRSCKAPLYDLAAHEDKVLSVDWTDTGLLLSGGADNKLYSYRYSPTTSHVGA 相同性解析による交差性が予測される生物種 |
| Full Name | WD repeat domain 12 |
| Calculated molecular weight | 423 aa, 48 kDa |
| Observed molecular weight | 47 kDa |
| GenBank accession number | BC008082 |
| Gene Symbol | WDR12 |
| Gene ID (NCBI) | 55759 |
| RRID | AB_3743085 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9GZL7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
WDR12 is a nucleolar WD-repeat scaffolding protein and an essential component of the PeBoW complex required for 60S ribosomal subunit maturation. By driving pre-rRNA processing, WDR12 supports cell growth and proliferation, while its disruption activates the p53-dependent nucleolar stress pathway. Upregulated in multiple cancers and genetically linked to cardiometabolic traits, WDR12 serves as a central regulator of ribosome biogenesis, cell-cycle control, and cellular homeostasis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for WDR12 antibody 33831-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |