Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 13676-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13676-1-AP (WDR1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 13676-1-AP
WB result of WDR1 antibody (13676-1-AP; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-WDR1 transfected HEK-293 cells.
WB analysis of rat skeletal muscle using 13676-1-AP
rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13676-1-AP (WDR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse skeletal muscle using 13676-1-AP
IP result of anti-WDR1 (IP:13676-1-AP, 3ug; Detection:13676-1-AP 1:500) with mouse skeletal muscle tissue lysate 3600ug.
IHC staining of human testis using 13676-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 13676-1-AP (WDR1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human colon using 13676-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13676-1-AP (WDR1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 13676-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 13676-1-AP (WDR1 antibody) at dilution of 1:100 (under 40x lens).

Featured Product
KD/KO Validated

WDR1 Polyclonal antibody

WDR1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 13676-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

WD repeat containing protein 1, NORI-1, NORI 1, AIP1, Actin-interacting protein 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, rat skeletal muscle tissue, mouse skeletal muscle, Jurkat cells, U-87 cells
Positive IP detected in	mouse skeletal muscle tissue
Positive IHC detected in	human colon tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 10 publications below
IHC	See 1 publications below

Product Information

13676-1-AP targets WDR1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4670 Product name: Recombinant human WDR1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC030541 Sequence: MPYEIKKVFASLPQVERGVSKIIGGDPKGNNFLYTNGKCVILRNIDDHSRFVNCVRFSPDGNRFATASADGQIYIYDGKTGEKVCALGGSKAHDGGIYAISWSPDSTHLLSASGDKTSKIWDVSVNSVVSTFPMGSTVLDQQLGCLWQKDHLLSVSLSGYINYLDRNNPSKPLHVIKGHSKSIQCLTVHKNGGKSYIYSGSHDGHINYWDSETGENDSFAGKGHTNQVSRMTVDESGQLISCSMDDTVRYTSLMLRDYSGQGVVKLDVQPKCVAVGPGGYAVVVCIGQIVLLKDQRKCFSIDNPGYEPEVVAVHPGGDTVAIGGVDGNVRLYSILGTTLKDEGKLLEAKG 相同性解析による交差性が予測される生物種
Full Name	WD repeat domain 1
Calculated molecular weight	606 aa, 66 kDa
Observed molecular weight	66-70 kDa
GenBank accession number	BC030541
Gene Symbol	WDR1
Gene ID (NCBI)	9948
RRID	AB_10697831
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75083
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

WDR1, which belongs to the WD repeat AIP1 family, also known as AIP1, is a 606 amino acid protein that localizes to the cytoskeleton. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, mostly including a trp-asp at the C-terminal end. WDR1 induces the disassembly of actin filaments in conjunction with ADF/cofilin family proteins (PMID:15629458, 27557945, 29751004). WDR1 mutations may impair the ability of WDR1 to regulate cofilin-mediated actin depolymerization, and are associated with periodic fever, immunodeficiency, and thrombocytopenia syndrome (PMID: 27557945,27994071,29751004).

Protocols

Product Specific Protocols
IHC protocol for WDR1 antibody 13676-1-AP	Download protocol
IP protocol for WDR1 antibody 13676-1-AP	Download protocol
WB protocol for WDR1 antibody 13676-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Death Dis AIP1-mediated actin disassembly is required for postnatal germ cell migration and spermatogonial stem cell niche establishment. Authors - J Xu View Article
human	WB	J Biol Chem Trp-Asp (WD) repeat domain 1 is Essential for Mouse Peri-implantation Development and Regulates Cofilin Phosphorylation. Authors - Yi Xiao View Article
mouse	WB	Am J Pathol A Cardiomyocyte-Specific Wdr1 Knockout Demonstrates Essential Functional Roles for Actin Disassembly during Myocardial Growth and Maintenance In Mice. Authors - Baiyin Yuan KO Validated View Article
human,mouse	WB	Mol Cells WD Repeat Domain 1 Deficiency Inhibits Neointima Formation in Mice Carotid Artery by Modulation of Smooth Muscle Cell Migration and Proliferation. Authors - JiSheng Hu KO Validated View Article
human	WB	Int J Biol Sci WDR1 Promotes Cell Growth and Migration and Contributes to Malignant Phenotypes of Non-small Cell Lung Cancer through ADF/cofilin-mediated Actin Dynamics. Authors - Baiyin Yuan KD Validated View Article
mouse	WB	Dev Biol WDR1-regulated actin dynamics is required for outflow tract and right ventricle development. Authors - Jisheng Hu KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13676-1-AP

WB analysis of HEK-293 using 13676-1-AP

WB analysis of rat skeletal muscle using 13676-1-AP

IP experiment of mouse skeletal muscle using 13676-1-AP

IHC staining of human testis using 13676-1-AP

IHC staining of human colon using 13676-1-AP

IHC staining of human testis using 13676-1-AP

WDR1 Polyclonal antibody

WDR1 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

AIP1-mediated actin disassembly is required for postnatal germ cell migration and spermatogonial stem cell niche establishment.

Trp-Asp (WD) repeat domain 1 is Essential for Mouse Peri-implantation Development and Regulates Cofilin Phosphorylation.

A Cardiomyocyte-Specific Wdr1 Knockout Demonstrates Essential Functional Roles for Actin Disassembly during Myocardial Growth and Maintenance In Mice.

WD Repeat Domain 1 Deficiency Inhibits Neointima Formation in Mice Carotid Artery by Modulation of Smooth Muscle Cell Migration and Proliferation.

WDR1 Promotes Cell Growth and Migration and Contributes to Malignant Phenotypes of Non-small Cell Lung Cancer through ADF/cofilin-mediated Actin Dynamics.

WDR1-regulated actin dynamics is required for outflow tract and right ventricle development.