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Target

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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

WDFY3 Polyclonal antibody

WDFY3 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55009-1-AP

Synonyms

ALFY, Autophagy linked FYVE protein, KIAA0993, WDFY3, ZFYVE25

Validation Data Gallery

Applications:
  • IHC staining of rat brain using 55009-1-AP

    IHC staining of rat brain using 55009-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 55009-1-AP (WDFY3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 55009-1-AP

    IHC staining of mouse brain using 55009-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55009-1-AP (WDFY3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 55009-1-AP

    IHC staining of mouse brain using 55009-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55009-1-AP (WDFY3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 55009-1-AP

    IHC staining of human breast cancer using 55009-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 55009-1-AP (WDFY3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 55009-1-AP

    IHC staining of human breast cancer using 55009-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 55009-1-AP (WDFY3 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human breast cancer using 55009-1-AP

    IHC staining of human breast cancer using 55009-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 55009-1-AP (WDFY3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 55009-1-AP

    IHC staining of human breast cancer using 55009-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 55009-1-AP (WDFY3 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of mouse brain using 55009-1-AP

    IHC staining of mouse brain using 55009-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55009-1-AP (WDFY3 Antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 55009-1-AP

    IHC staining of mouse brain using 55009-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55009-1-AP (WDFY3 Antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inrat brain tissue, mouse brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55009-1-AP targets WDFY3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name WD repeat and FYVE domain containing 3
Calculated molecular weight 395 kDa
GenBank accession numberNM_014991
Gene symbol WDFY3
Gene ID (NCBI) 23001
RRIDAB_10863126
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WDFY3, also named as KIAA0993 and Alfy, is a protein which contains WD repeats and an FYVE domain. Multiple alternatively spliced transcript variants have been found for WDFY3, but the full-length nature of some variants has not been defined. The antibody is specific to WDFY3.The band 50kd in WB is a splicing form of WDFY3.

Protocols

Product Specific Protocols
IHC protocol for WDFY3 antibody 55009-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC,IF

Cardiovasc Res

WD40 repeat and FYVE domain containing 3 (Wdfy3) is essential for cardiac development.

Authors - Shasha Zhang
  • KO Validated
View Article
mouseIHC, IF

In Vitro Cell Dev Biol Anim

Autophagy-linked FYVE protein (Alfy) promotes autophagic removal of misfolded proteins involved in amyotrophic lateral sclerosis (ALS).

Authors - Huihui Han
View Article