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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using WBP2 Monoclonal antibody (66585-1-Ig)

    WB analysis of HEK-293 using 66585-1-Ig (same clone as 66585-1-PBS)

    WB result of WBP2 antibody (66585-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WBP2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66585-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using WBP2 Monoclonal antibody (66585-1-Ig)

    WB analysis of HeLa using 66585-1-Ig (same clone as 66585-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66585-1-Ig (WBP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66585-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using WBP2 Monoclonal antibody (66585-1-Ig)

    WB analysis of NIH/3T3 using 66585-1-Ig (same clone as 66585-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66585-1-Ig (WBP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using WBP2 Monoclonal antibody (66585-1-Ig)

    IHC staining of mouse testis using 66585-1-Ig (same clone as 66585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66585-1-Ig (WBP2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using WBP2 Monoclonal antibody (66585-1-Ig)

    IHC staining of mouse testis using 66585-1-Ig (same clone as 66585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66585-1-Ig (WBP2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66585-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using WBP2 Monoclonal antibody (66585-1-Ig)

    IF Staining of HeLa using 66585-1-Ig (same clone as 66585-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using WBP2 antibody (66585-1-Ig, Clone: 2B4G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66585-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using WBP2 Monoclonal antibody (66585-1-Ig)

    IF Staining of HeLa using 66585-1-Ig (same clone as 66585-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WBP2 antibody (66585-1-Ig, Clone: 2B4G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66585-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

WBP2 Monoclonal antibody, PBS Only

WBP2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66585-1-PBS
Clone No.2B4G7

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

WBP 2, WBP2, WW domain binding protein 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66585-1-PBS targets WBP2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag2661

Product name: Recombinant human WBP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-261 aa of BC010616

Sequence: MALNKNHSEGGGVIVNNTESILMSYDHVELTFNDMKNVPEAFKGTKKGTVYLTPYRVIFLSKGKDAMQSFMMPFYLMKDCEIKQPVFGANYIKGTVKAEAGGGWEGSASYKLTFTAGGAIEFGQRMLQVASQASRGEVPSGAYGYSYMPSGAYVYPPPVANGMYPCPPGYPYPPPPPEFYPGPPMMDGAMGYVQPPPPPYPGPMEPPVSGPDVPSTPAAEAKAAEAAASAYYNPGNPHNVYMPTSQPPPPPYYPPEDKKTQ

相同性解析による交差性が予測される生物種
Full Name WW domain binding protein 2
Calculated molecular weight 261 aa, 28 kDa
Observed molecular weight 30-40 kDa
GenBank accession numberBC010616
Gene Symbol WBP2
Gene ID (NCBI) 23558
RRIDAB_2881945
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ969T9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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