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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WBP11 Polyclonal antibody (10316-1-AP)

    WB analysis using 10316-1-AP (same clone as 10316-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10316-1-AP (WBP11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10316-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using WBP11 Polyclonal antibody (10316-1-AP)

    IP experiment of mouse heart using 10316-1-AP (same clone as 10316-1-PBS)

    IP result of anti-WBP11 (IP:10316-1-AP, 3ug; Detection:10316-1-AP 1:500) with mouse heart tissue lysate 6000ug. This data was developed using the same antibody clone with 10316-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using WBP11 Polyclonal antibody (10316-1-AP)

    IHC staining of human breast cancer using 10316-1-AP (same clone as 10316-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10316-1-AP (WBP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10316-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using WBP11 Polyclonal antibody (10316-1-AP)

    IHC staining of human breast cancer using 10316-1-AP (same clone as 10316-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10316-1-AP (WBP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10316-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using WBP11 Polyclonal antibody (10316-1-AP)

    IF Staining of HeLa using 10316-1-AP (same clone as 10316-1-PBS)

    Immunofluorescent analysis of HeLa cells using 10316-1-AP (WBP11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10316-1-PBS in a different storage buffer formulation.

WBP11 Polyclonal antibody, PBS Only

WBP11 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10316-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10316-1-PBS targets WBP11 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0366

Product name: Recombinant human WBP11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 170-379 aa of BC001621

Sequence: TSAYGPPTRAVSILPLLGHGVPRLPPGRKPPGPPPGPPPPQVVQMYGRKVGFALDLPPRRRDEDMLYSPELAQRGHDDDVSSTSEDDGYPEDMDQDKHDDSTDDSDTDKSDGESDGDEFVHRDNGERDNNEEKKSGLSVRFADMPGKSRKKKKNMKELTPLQAMMLRMAGQEIPEEGREVEEFSEDDDEDDSDDSEAEKQSQKQHKEESH

相同性解析による交差性が予測される生物種
Full Name WW domain binding protein 11
Calculated molecular weight 70 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC001621
Gene Symbol WBP11
Gene ID (NCBI) 51729
RRIDAB_10694664
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2W2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

WBP11 (WW domain-binding protein 11) is a protein that in humans, is encoded by the WBP11 gene. The function of WBP11 is to play a role in the regulation of pre-mRNA processing. More specifically, this nuclear protein, colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. The predicted MW of this protein is 70 kDa, and this antibody specially recognises the 70 kDa protein.

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