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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using WASF4-Specific Polyclonal antibody (19755-1-AP)

    WB analysis of mouse brain using 19755-1-AP (same clone as 19755-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19755-1-AP (WASF4-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19755-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using WASF4-Specific Polyclonal antibody (19755-1-AP)

    WB analysis of mouse brain using 19755-1-AP (same clone as 19755-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19755-1-AP (WASF4-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19755-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using WASF4-Specific Polyclonal antibody (19755-1-AP)

    IF Staining of HepG2 using 19755-1-AP (same clone as 19755-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using WASF4 antibody 19755-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 19755-1-PBS in a different storage buffer formulation.

WASF4-Specific Polyclonal antibody, PBS Only

WASF4-Specific Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 19755-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19755-1-PBS targets WASF4-Specific in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name WAS protein family, member 4
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberQ8IV90
Gene Symbol WASF4
Gene ID (NCBI) 644739
RRIDAB_10646483
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

WASF4, also named as SCAR2, belongs to the SCAR/WAVE family. WASF4 is a downstream effector molecule which involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. The antibody is specific to WASF4.

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