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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WASF2 Polyclonal antibody (31442-1-AP)

    WB analysis using 31442-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31442-1-AP (WASF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U2OS cells using WASF2 Polyclonal antibody (31442-1-AP)

    WB analysis of U2OS using 31442-1-AP

    WB result of WASF2 antibody (31442-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WASF2 transfected U2OS cells.

  • Immunohistochemistry (IHC) staining of human placenta tissue using WASF2 Polyclonal antibody (31442-1-AP)

    IHC staining of human placenta using 31442-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31442-1-AP (WASF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using WASF2 Polyclonal antibody (31442-1-AP)

    IF Staining of U2OS using 31442-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using WASF2 antibody (31442-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

WASF2 Polyclonal antibody

WASF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31442-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Protein WAVE 2, SCAR2, WAS protein family, member 2, WASP family protein member 2, WAVE2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMDA-MB-231 cells, U2OS cells, K-562 cells, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31442-1-AP targets WASF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag35663

Product name: Recombinant human WASF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 182-315 aa of NM_006990.4

Sequence: KKDNPNRGNVNPRKIKTRKEEWEKMKMGQEFVESKEKLGTSGYPPTLVYQNGSIGCVENVDASSYPPPPQSDSASSPSPSFSEDNLPPPPAEFSYPVDNQRGSGLAGPKRSSVVSPSHPPPAPPLGSPPGPKPG

相同性解析による交差性が予測される生物種
Full Name WAS protein family, member 2
Calculated molecular weight54kDa，498aa
Observed molecular weight80 kDa
GenBank accession numberNM_006990.4
Gene Symbol WASF2
Gene ID (NCBI) 10163
RRIDAB_3669985
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9Y6W5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WASF2 (WASp family Verprolin-homologous protein-2), also known as WAVE2, a member of the Wiskott-Aldrich syndrome protein (WASp) family of actin nucleation promoting factors, is a downstream effector molecule that implicates the signal transduction from small GTPases to the actin cytoskeleton, which can elongate the cell movement mode (PMID: 35849030,28332566). Moreover, WASF2 promoted the invasiveness of cancer cells by binding to actin cytoskeletal protein alpha-actinin 4 (ACTN4) (PMID: 30353690). The calculated MW of WASF2 is 54 kDa, with an isoform of 32 kDa, but the observed MW is about 80 kDa, which may be due to post-translational modification (PMID: 35849030,28332566,21383498,17389688,18362183,38391912).

Protocols

Product Specific Protocols
IF protocol for WASF2 antibody 31442-1-APDownload protocol
IHC protocol for WASF2 antibody 31442-1-APDownload protocol
WB protocol for WASF2 antibody 31442-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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