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Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WASF1 Polyclonal antibody (32584-1-AP)

    WB analysis using 32584-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32584-1-AP (WASF1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using WASF1 Polyclonal antibody (32584-1-AP)

    IP experiment of HEK-293 using 32584-1-AP

    IP result of anti-WASF1 (IP:32584-1-AP, 4ug; Detection:32584-1-AP 1:3000) with HEK-293 cells lysate 1800 ug.

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WASF1 Polyclonal antibody

WASF1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 32584-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

Actin-binding protein WASF1, KIAA0269, Protein WAVE-1, SCAR1, Verprolin homology domain-containing protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32584-1-AP targets WASF1 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WASF1 fusion protein Ag38505 相同性解析による交差性が予測される生物種
Full Name WAS protein family, member 1
Calculated molecular weight62kDa，559aa
Observed molecular weight75 kDa
GenBank accession numberNM_003931.2
Gene Symbol WASF1
Gene ID (NCBI) 8936
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ92558
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WASF1 (Wiskott-Aldrich Syndrome Protein Family Member 1) is a gene encoding an actin-binding protein that belongs to the Wiskott-Aldrich syndrome protein family. It is primarily involved in regulating the reorganization of the actin cytoskeleton in cells, and is essential for processes such as cell morphology, motility, and signaling. WASF1 promotes actin polymerization by interacting with the Arp2/3 complex, thereby affecting cell deformation and migration. WASF1 is expressed in a variety of cell types, including tissues such as brain and testis. Its aberrant expression is associated with a variety of diseases, such as tumor invasion and metastasis.

Protocols

Product Specific Protocols
WB protocol for WASF1 antibody 32584-1-APDownload protocol
IP protocol for WASF1 antibody 32584-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols