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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WASP Recombinant antibody (84714-2-RR)

    WB analysis using 84714-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84714-2-RR (WASP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84714-2-RR

    Affinity and Kinetic Characterization of 84714-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84714-2-RR against Human WASP were performed. The affinity constant is 1.88 nM.

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WASP Recombinant antibody

WASP Recombinant Antibody for WB, ELISA
Cat No. 84714-2-RR
Clone No.242074E4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Wiskott-Aldrich syndrome protein, WAS, Actin nucleation-promoting factor WAS, 242074E4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, TF-1 cells, HL-60 cells, Raji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84714-2-RR targets WASP in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen WASP fusion protein Ag1444 相同性解析による交差性が予測される生物種
Full Name Wiskott-Aldrich syndrome (eczema-thrombocytopenia)
Calculated molecular weight 53 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC012738
Gene Symbol WASP
Gene ID (NCBI) 7454
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42768
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Wiskott-Aldrich Syndrome protein (WASP) regulates actin cytoskeleton in hematopoietic cells and defects in WASP cause Wiskott-Aldrich Syndrome (WAS), an X-linked immunodeficiency and autoimmunity disorder of childhood.

Protocols

Product Specific Protocols
WB protocol for WASP antibody 84714-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols