Validation Data Gallery
Tested Applications
Positive WB detected in | HL-60 cells, K-562 cells, mouse spleen tissue, Jurkat cells, Raji cells |
Positive IP detected in | Raji cells, rat spleen tissue |
Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 4 publications below |
IP | See 1 publications below |
RIP | See 1 publications below |
Product Information
10987-1-AP targets WASP in WB, IHC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | WASP fusion protein Ag1444 相同性解析による交差性が予測される生物種 |
Full Name | Wiskott-Aldrich syndrome (eczema-thrombocytopenia) |
Calculated molecular weight | 53 kDa |
Observed molecular weight | 55-62 kDa |
GenBank accession number | BC012738 |
Gene Symbol | WASP |
Gene ID (NCBI) | 7454 |
RRID | AB_2304546 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P42768 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Wiskott-Aldrich Syndrome protein (WASP) regulates actin cytoskeleton in hematopoietic cells and defects in WASP cause Wiskott-Aldrich Syndrome (WAS), an X-linked immunodeficiency and autoimmunity disorder of childhood.
Protocols
Product Specific Protocols | |
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WB protocol for WASP antibody 10987-1-AP | Download protocol |
IHC protocol for WASP antibody 10987-1-AP | Download protocol |
IP protocol for WASP antibody 10987-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Allergy Clin Immunol Dedicator of cytokinesis protein 2 couples with lymphoid enhancer-binding factor 1 to regulate expression of CD21 and B-cell differentiation. | ||
Nat Commun WASp modulates RPA function on single-stranded DNA in response to replication stress and DNA damage. | ||
J Exp Clin Cancer Res miR-182 suppresses invadopodia formation and metastasis in non-small cell lung cancer by targeting cortactin gene.
| ||
J Exp Clin Cancer Res CDC42-interacting protein 4 promotes metastasis of nasopharyngeal carcinoma by mediating invadopodia formation and activating EGFR signaling. | ||
Mol Cancer Res LINC00869 Promotes Hepatocellular Carcinoma Metastasis via Protrusion Formation |