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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

WARS2 Polyclonal antibody

WARS2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13944-1-AP

Synonyms

(Mt)TrpRS, TrpRS, Tryptophan tRNA ligase, WARS2

Validation Data Gallery

Applications:
  • WB analysis of human liver using 13944-1-AP

    WB analysis of human liver using 13944-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13944-1-AP (WARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 13944-1-AP

    WB analysis of mouse brain using 13944-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13944-1-AP (WARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman liver tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Sample-dependent, check data in validation data gallery

Product Information

13944-1-AP targets WARS2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WARS2 fusion protein Ag5024 相同性解析による交差性が予測される生物種
Full Name tryptophanyl tRNA synthetase 2, mitochondrial
Calculated molecular weight 40 kDa
Observed molecular weight 60 kDa/55 kDa
GenBank accession numberBC044575
Gene symbol WARS2
Gene ID (NCBI) 10352
RRIDAB_2304541
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WARS2(tryptophan--tRNA ligase, mitochondrial) is also called mitochondrial TRPRS and belongs to the class-I aminoacyl-tRNA synthetase family. The gene encodes the protein containing 360-amino acid ,which has an N-terminal mitochondrial targeting signal that is cleaved after residue 18. WARS2 has characteristics of a class I aminoacyl-tRNA synthetase, including a classical Rossmann fold(PMID:15779907). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for WARS2 antibody 13944-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols