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Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using Vitamin D binding protein Monoclonal antibody (66175-1-Ig)

    WB analysis of human testis using 66175-1-Ig (same clone as 66175-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66175-1-Ig (Vitamin D binding protein antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66175-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Vitamin D binding protein Monoclonal antibody (66175-1-Ig)

    IHC staining of human liver using 66175-1-Ig (same clone as 66175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66175-1-Ig (Vitamin D binding protein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66175-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Vitamin D binding protein Monoclonal antibody (66175-1-Ig)

    IHC staining of human colon cancer using 66175-1-Ig (same clone as 66175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66175-1-Ig (Vitamin D binding protein antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66175-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Vitamin D binding protein Monoclonal antibody (66175-1-Ig)

    IHC staining of human liver cancer using 66175-1-Ig (same clone as 66175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66175-1-Ig (Vitamin D binding protein antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66175-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Vitamin D binding protein Monoclonal antibody (66175-1-Ig)

    IF Staining of HepG2 using 66175-1-Ig (same clone as 66175-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Vitamin D binding protein antibody (66175-1-Ig, Clone: 1E4D10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66175-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-937 cells using Vitamin D binding protein Monoclonal antibody (66175-1-Ig)

    FC experiment of U-937 using 66175-1-Ig (same clone as 66175-1-PBS)

    1X10^6 U-937 cells were intracellularly stained with 0.4 ug Anti-Human Vitamin D binding protein (66175-1-Ig, Clone:1E4D10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66175-1-PBS in a different storage buffer formulation.

Vitamin D binding protein Monoclonal antibody, PBS Only

Vitamin D binding protein Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66175-1-PBS
Clone No.1E4D10

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

GC, VDBP,GC, DBP, DBP/GC, DBP-maf

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66175-1-PBS targets Vitamin D binding protein in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag9803

Product name: Recombinant human VDBP,GC protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 120-474 aa of BC057228

Sequence: KLCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKEYANQFMWEYSTNYGQAPLSLLVSYTKSYLSMVGSCCTSASPTVCFLKERLQLKHLSLLTTLSNRVCSQYAAYGEKKSRLSNLIKLAQKVPTADLEDVLPLAEDITNILSKCCESASEDCMAKELPEHTVKLCDNLSTKNSKFEDCCQEKTAMDVFVCTYFMPAAQLPELPDVELPTNKDVCDPGNTKVMDKYTFELSRRTHLPEVFLSKVLEPTLKSLGECCDVEDSTTCFNAKGPLLKKELSSFIDKGQELCADYSENTFTEYKKKLAERLKAKLPDATPTELAKLVNKRSDFASNCCSINSPPLYCDSEIDAELKNIL

相同性解析による交差性が予測される生物種
Full Name group-specific component (vitamin D binding protein)
Calculated molecular weight 474 aa, 53 kDa
Observed molecular weight 52-58 kDa
GenBank accession numberBC057228
Gene Symbol Vitamin D binding protein
Gene ID (NCBI) 2638
RRIDAB_2881570
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP02774
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Vitamin D binding protein is a sparsely glycosylated serum protein responsible for highly specific binding and tissue-specific delivery of vitamin D and its metabolites. In addition, it is also an actin scavenger, and is the precursor to the immunomodulatory protein, Gc-MAF. Vitamin D binding protein has been proposed to have significant roles in C5a chemotaxis, osteoclast development and possibly in macrophage activation/recruitment.

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