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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Vinculin Recombinant monoclonal antibody (86930-1-RR)

    WB analysis using 86930-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86930-1-RR (Vinculin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Vinculin Recombinant monoclonal antibody (86930-1-RR)

    IHC staining of mouse heart using 86930-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86930-1-RR (Vinculin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Vinculin Recombinant monoclonal antibody (86930-1-RR)

    IHC staining of mouse heart using 86930-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86930-1-RR (Vinculin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using Vinculin Recombinant monoclonal antibody (86930-1-RR)

    IF Staining of mouse colon using 86930-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Vinculin antibody (86930-1-RR, Clone: 251919D10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using Vinculin Recombinant monoclonal antibody (86930-1-RR)

    IF Staining of mouse colon using 86930-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Vinculin antibody (86930-1-RR, Clone: 251919D10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Vinculin Recombinant monoclonal antibody (86930-1-RR)

    IF Staining of A549 using 86930-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Vinculin antibody (86930-1-RR, Clone: 251919D10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Vinculin Recombinant monoclonal antibody (86930-1-RR)

    IF Staining of A549 using 86930-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Vinculin antibody (86930-1-RR, Clone: 251919D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 86930-1-RR

    Affinity and Kinetic Characterization of 86930-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86930-1-RR against Human Vinculin were performed. The affinity constant is below 1 pM.

Vinculin Recombinant monoclonal antibody

Vinculin Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 86930-1-RR
Clone No.251919D10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

CMD1W, Metavinculin, MV, MVCL, VCL

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, L02 cells, HUVEC cells, HGC-27 cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse colon tissue
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86930-1-RR targets Vinculin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag24942

Product name: Recombinant human Vinculin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 777-1066 aa of BC039174

Sequence: PKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

相同性解析による交差性が予測される生物種
Full Name vinculin
Calculated molecular weight 1133 aa, 124 kDa
Observed molecular weight124 kDa
GenBank accession numberBC039174
Gene Symbol Vinculin
Gene ID (NCBI) 7414
RRIDAB_3745213
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP18206
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein that is involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein that is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is a muscle-specific protein.

Protocols

Product Specific Protocols
IF protocol for Vinculin antibody 86930-1-RRDownload protocol
IHC protocol for Vinculin antibody 86930-1-RRDownload protocol
WB protocol for Vinculin antibody 86930-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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