• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Vinculin Monoclonal antibody (66305-2-Ig)

    WB analysis using 66305-2-Ig (same clone as 66305-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Vinculin Monoclonal antibody (66305-2-Ig)

    WB analysis using 66305-2-Ig (same clone as 66305-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Vinculin Monoclonal antibody (66305-2-Ig)

    WB analysis using 66305-2-Ig (same clone as 66305-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using Vinculin Monoclonal antibody (66305-2-Ig)

    IHC staining of human ovary cancer using 66305-2-Ig (same clone as 66305-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66305-2-Ig (Vinculin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using Vinculin Monoclonal antibody (66305-2-Ig)

    IHC staining of human ovary cancer using 66305-2-Ig (same clone as 66305-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66305-2-Ig (Vinculin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using Vinculin Monoclonal antibody (66305-2-Ig)

    IF Staining of hTERT-RPE1 using 66305-2-Ig (same clone as 66305-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using Vinculin antibody (66305-2-Ig, Clone: 3A11F8 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66305-2-PBS in a different storage buffer formulation.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Vinculin Monoclonal antibody, PBS Only

Vinculin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66305-2-PBS
Clone No.3A11F8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

3A11F8, CMD1W, Metavinculin, MV, MVCL

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66305-2-PBS targets Vinculin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Vinculin fusion protein Ag24946 相同性解析による交差性が予測される生物種
Full Name vinculin
Calculated molecular weight 1133 aa, 124 kDa
Observed molecular weight124 kDa
GenBank accession numberBC039174
Gene Symbol Vinculin
Gene ID (NCBI) 7414
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP18206
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein that is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.