Vinculin Polyclonal antibody

Vinculin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 26520-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

CMD1W, Metavinculin, MV, MVCL, VCL

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, MDCK cells, NIH/3T3 cells, PC-12 cells, mouse heart tissue, rat heart tissue
Positive IHC detected inmouse colon tissue, human breast cancer tissue, human prostate cancer tissue, human skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, A549 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:60000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26520-1-AP targets Vinculin in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse, rat, pig, rabbit, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag24942

Product name: Recombinant human Vinculin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 777-1066 aa of BC039174

Sequence: PKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

相同性解析による交差性が予測される生物種
Full Name vinculin
Calculated molecular weight 1133 aa, 124 kDa
Observed molecular weight 124 kDa
GenBank accession numberBC039174
Gene Symbol Vinculin
Gene ID (NCBI) 7414
RRIDAB_2868558
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP18206
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

Protocols

Product Specific Protocols
WB protocol for Vinculin antibody 26520-1-APDownload protocol
IHC protocol for Vinculin antibody 26520-1-APDownload protocol
IF protocol for Vinculin antibody 26520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cell Metab

Electron transport chain inhibition increases cellular dependence on purine transport and salvage

Authors - Zheng Wu
humanWB

Blood

Epigenetic Regulation of Non-canonical Menin Targets Modulates Menin Inhibitor Response in Acute Myeloid Leukemia

Authors - Xinyue Zhou
humanWB,IF

ACS Nano

Polyethylenimine-Modified Mesoporous Silica Nanoparticles Induce a Survival Mechanism in Vascular Endothelial Cells via Microvesicle-Mediated Autophagosome Release

Authors - Lu Zhang
humanWB

Mol Cell

Type III CRISPR-mediated flexible RNA excision with engineered guide RNAs

Authors - Yuanfan Sun
mouseIF

Adv Sci (Weinh)

Highly Effective and Noninvasive Near-Infrared Eradication of a Staphylococcus aureus Biofilm on Implants by a Photoresponsive Coating within 20 Min.

Authors - Mu Li
rabbitWB,IF

Adv Sci (Weinh)

Biomimetic Convex Implant for Corneal Regeneration Through 3D Printing

Authors - Yingni Xu
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