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Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using Villin Monoclonal antibody (66096-1-Ig)

    WB analysis of human kidney using 66096-1-Ig (same clone as 66096-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 66096-1-Ig (Villin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human colon tissue using Villin Monoclonal antibody (66096-1-Ig)

    WB analysis of human colon using 66096-1-Ig (same clone as 66096-1-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 66096-1-Ig (Villin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human small intestine tissue using Villin Monoclonal antibody (66096-1-Ig)

    WB analysis of human small intestine using 66096-1-Ig (same clone as 66096-1-PBS)

    human small intestine tissue were subjected to SDS PAGE followed by western blot with 66096-1-Ig (Villin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using Villin Monoclonal antibody (66096-1-Ig)

    IP experiment of mouse kidney using 66096-1-Ig (same clone as 66096-1-PBS)

    IP result of anti-Villin (IP:66096-1-Ig, 4ug; Detection:66096-1-Ig 1:1000) with mouse kidney tissue lysate 6000ug. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Villin Monoclonal antibody (66096-1-Ig)

    IHC staining of human colon using 66096-1-Ig (same clone as 66096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66096-1-Ig (Villin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Villin Monoclonal antibody (66096-1-Ig)

    IHC staining of human colon using 66096-1-Ig (same clone as 66096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66096-1-Ig (Villin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Villin Monoclonal antibody (66096-1-Ig)

    IF Staining of HepG2 using 66096-1-Ig (same clone as 66096-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66096-1-Ig (Villin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

Villin Monoclonal antibody, PBS Only

Villin Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66096-1-PBS
Clone No.2B7B9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

VIL1, 2B7B9, D2S1471, VIL, villin 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66096-1-PBS targets Villin in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag9637

Product name: Recombinant human VIL1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC017303

Sequence: MTKLSAQVKGSLNITTPGLQIWRIEAMQMVPVPSSTFGSFFDGDCYIILAIHKTASSLSYDIHYWIGQDSSLDEQGAAAIYTTQMDDFLKGRAVQHREVQGNESEAFRGYFKQGLVIRKGGVASGMKHVETNSYDVQRLLHVKGKRNVVAGEVEMSWKSFNRGDVFLLDLGKLIIQWNGPESTRMERLRGMTLAKEIRDQERGGRTYVGVVDGENELASPKLMEVMNHVLGKRRELKAAVPDTVVEPALKAALKLYHVSDSEGNLVVREVATRPLTQDLLSHEDCYILDQGGLKIYVWKGKKANEQEKKGAMSHALNFIKAKQYPPSTQVEVQNDGAESAVFQQLFQKWT

相同性解析による交差性が予測される生物種
Full Name villin 1
Calculated molecular weight827aa,93 kDa; 826aa,93 kDa
Observed molecular weight 93-95 kDa
GenBank accession numberBC017303
Gene Symbol Villin
Gene ID (NCBI) 7429
RRIDAB_2881495
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP09327
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Villin 1 (VIL1) is a 95-kDa F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border, like epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. In addition, VIL1 is a useful diagnostic marker for of various cancer, like cervical and endometrial adenocarcinomas, renal cell carcinoma. VIL1 was recently identified as a novel biomarker predictive for postoperative recurrence and poorer prognosis of high serum AFP associated HCC.

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