Product Information
Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI method) provides dual fluorescence staining for the detection of living and dead cells. The two probes in the kit eflect cell viability by measuring intracellular esterase activity and plasma membrane integrity. This kit can be used for fluorescence microscopes, flow cytometers, microplate readers and other fluorescence detection systems.
This kit can be used for most eukaryotic mammalian samples, but not to fungi and yeast.
Components | 150T | 300T |
A. Calcein AM (4 mM in anhydrous DMSO) | 50 uL | 100 uL |
B. Propidium (PI) (1.5 mM in H2O) | 250 uL | 500 uL |
Note: The number of times(T) of this kit is specified according to the usage of 0.5mL working solution for one sample of flow cytometry.
Storage
Store at -20°C. Avoid exposure to light. Stable for 24 months after shipment.
Cautions
1. Fluorescent dyes all have quenching problems.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
Parameters
CalceinAM：Ex/Em=494/517nm
PI: Ex/Em=535/617nm (with DNA)
Cited in Article as
pf00007, Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI Method), Proteintech, IL, USA
Documentation
| SDS |
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| Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI Method) Datasheet |
| Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI Method) Datasheet |
| Datasheet |
|---|
| Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI Method) Datasheet |
Publications
| Application | Title |
|---|---|
Adv Mater Matrix Viscoelasticity Controls Differentiation of Human Blood Vessel Organoids into Arterioles and Promotes Neovascularization in Myocardial Infarction | |
Adv Mater Harmonizing Thickness And Permeability in Bone Tissue Engineering: A Novel Silk Fibroin Membrane Inspired by Spider Silk Dynamics | |
Bioact Mater Construction of tissue-engineered vascular grafts with enhanced patency by integrating heparin, cell-adhesive peptide, and carbon monoxide nanogenerators into acellular blood vessels | |
Bioact Mater Differentiation of arterioles and capillaries in human blood vessel organoids with decellularized splenic matrix. | |
J Extracell Vesicles TMTP1-Modified Small Extracellular Vesicles Target BRAF Mutation in Anaplastic Thyroid Cancer Reversing Vemurafenib Resistance With CRISPR/Cas9 Delivery. | |
Nat Commun In situ osteogenic activation of mesenchymal stem cells by the blood clot biomimetic mechanical microenvironment |