Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, NIH/3T3 cells, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 33 publications below |
| IHC | See 12 publications below |
| IF | See 5 publications below |
Product Information
26157-1-AP targets VEGFA in WB, IHC, IF, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24084 Product name: Recombinant mouse Vegfa protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 27-214 aa of NM_001287056 Sequence: APTTEGEQKSHEVIKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTSESNITMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPEKKSVRGKGKGQKRKRKKSRFKSWSVHCEPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR 相同性解析による交差性が予測される生物種 |
| Full Name | vascular endothelial growth factor A |
| Observed molecular weight | 45 kDa |
| GenBank accession number | NM_001287056 |
| Gene Symbol | Vegfa |
| Gene ID (NCBI) | 22339 |
| RRID | AB_2880408 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q00731 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Vegfa is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis.It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. It may play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for VEGFA antibody 26157-1-AP | Download protocol |
| WB protocol for VEGFA antibody 26157-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Nano Flowerbed-Inspired Mg-Loaded Scaffold Accelerates Critical-Sized Bone-Defect Repair by Reprogramming the Microenvironment. | ||
ACS Nano Natural Polyphenol-Functionalized Schwann Cell-Derived Exosomes as a Temporal Neuromodulation Strategy for Diabetic Periodontitis Therapy. | ||
J Nanobiotechnology Protocatechualdehyde-functionalized BMSC-derived exosomes orchestrate calcium signaling in the immunoinflammatory microenvironment to promote periodontal regeneration. | ||
J Nanobiotechnology A sequential dual-drug delivery system for multi-target inhibition of subretinal fibrosis. | ||
Adv Sci (Weinh) A DNA Tetrahedron Delivery Asiatic Acid to Reprogram Mitochondrial Metabolism for Promoting Bone Regeneration via STAT3 Phosphorylation. | ||
Adv Sci (Weinh) Ultrasound-Activated Bifunctional Piezoelectric Hydrogel Dressings Promote Infected Wound Healing via Regulating Angiogenesis and Lymphangiogenesis. |