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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VWA8 Polyclonal antibody (31348-1-AP)

    WB analysis using 31348-1-AP (same clone as 31348-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 31348-1-AP (KIAA0564 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31348-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using VWA8 Polyclonal antibody (31348-1-AP)

    IHC staining of mouse kidney using 31348-1-AP (same clone as 31348-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31348-1-AP (VWA8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31348-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using VWA8 Polyclonal antibody (31348-1-AP)

    IHC staining of mouse kidney using 31348-1-AP (same clone as 31348-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31348-1-AP (VWA8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31348-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using VWA8 Polyclonal antibody (31348-1-AP)

    IF Staining of U2OS using 31348-1-AP (same clone as 31348-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using VWA8 antibody (31348-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 31348-1-PBS in a different storage buffer formulation.

VWA8 Polyclonal antibody, PBS Only

VWA8 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31348-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

RP97, P7BP2, KIAA0564

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31348-1-PBS targets VWA8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag35404

Product name: Recombinant human KIAA0564 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1700-1905 aa of NM_015058.1

Sequence: ELEPQLGSPQQKPKRLRLVVDVSGSMYRFNRMDGRLERTMEAVCMVMEAFENYEEKFQYDIVGHSGDGYNIGLVPMNKIPKDNKQRLEILKTMHAHSQFCMSGDHTLEGTEHAIKEIVKEEADEYFVIVLSDANLSRYGIHPAKFAQILTRDPQVNAFAIFIGSLGDQATRLQRTLPAGRSFVAMDTKDIPQILQQIFTSTMLSSV

相同性解析による交差性が予測される生物種
Full Name KIAA0564
Calculated molecular weight215kDa，1905aa
Observed molecular weight204-215 kDa, 117 kDa
GenBank accession numberNM_015058.1
Gene Symbol KIAA0564
Gene ID (NCBI) 23078
RRIDAB_3669955
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDA3KMH1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

VWA8, also known as KIAA0564, is a mitochondrial matrix-targeted protein having a putative ATPase activity (PMID: 34660594). It has 3 isoforms with the MW of 215, 204, and 117 kDa.

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