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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using VWA2 Polyclonal antibody (18714-1-AP)

    WB analysis of A375 using 18714-1-AP (same clone as 18714-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 18714-1-AP (VWA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18714-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using VWA2 Polyclonal antibody (18714-1-AP)

    IHC staining of human colon using 18714-1-AP (same clone as 18714-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18714-1-AP (VWA2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 18714-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using VWA2 Polyclonal antibody (18714-1-AP)

    IHC staining of human colon using 18714-1-AP (same clone as 18714-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18714-1-AP (VWA2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 18714-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using VWA2 Polyclonal antibody (18714-1-AP)

    IHC staining of human colon cancer using 18714-1-AP (same clone as 18714-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 18714-1-AP (VWA2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18714-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using VWA2 Polyclonal antibody (18714-1-AP)

    IHC staining of human colon cancer using 18714-1-AP (same clone as 18714-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 18714-1-AP (VWA2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18714-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using VWA2 Polyclonal antibody (18714-1-AP)

    IF Staining of A375 using 18714-1-AP (same clone as 18714-1-PBS)

    Immunofluorescent analysis of A375 cells using 18714-1-AP (VWA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18714-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using VWA2 Polyclonal antibody (18714-1-AP)

    IF Staining of HEK-293 using 18714-1-AP (same clone as 18714-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using VWA2 antibody (18714-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 18714-1-PBS in a different storage buffer formulation.

VWA2 Polyclonal antibody, PBS Only

VWA2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18714-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

von Willebrand factor A domain-containing protein 2, NET42, CCSP 2, AMACO, A domain-containing protein similar to matrilin and collagen

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18714-1-PBS targets VWA2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name von Willebrand factor A domain containing 2
Calculated molecular weight 82 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC128588
Gene Symbol VWA2
Gene ID (NCBI) 340706
RRIDAB_10598772
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5GFL6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

VWA2, also named as AMACO and CCSP2, is a novel candidate serological marker of colon neoplasia. VWA2 is found in the media of variesy of established cell lines of both fibroblast and epithelial origin. This antibody can recognize all the 3 isoforms of VWA2.

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