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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Vitronectin/S-protein Monoclonal antibody (66398-1-Ig)

    WB analysis of human plasma using 66398-1-Ig (same clone as 66398-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66398-1-Ig (VTN Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig plasma using Vitronectin/S-protein Monoclonal antibody (66398-1-Ig)

    WB analysis of pig plasma using 66398-1-Ig (same clone as 66398-1-PBS)

    pig plasma were subjected to SDS PAGE followed by western blot with 66398-1-Ig (VTN Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit serum using Vitronectin/S-protein Monoclonal antibody (66398-1-Ig)

    WB analysis of rabbit serum using 66398-1-Ig (same clone as 66398-1-PBS)

    rabbit serum were subjected to SDS PAGE followed by western blot with 66398-1-Ig (VTN Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using Vitronectin/S-protein Monoclonal antibody (66398-1-Ig)

    IHC staining of human testis using 66398-1-Ig (same clone as 66398-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66398-1-Ig (VTN Antibody) at dilution of 1:350 (under 10x lens). This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using Vitronectin/S-protein Monoclonal antibody (66398-1-Ig)

    IHC staining of human testis using 66398-1-Ig (same clone as 66398-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66398-1-Ig (VTN Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Vitronectin/S-protein Monoclonal antibody (66398-1-Ig)

    IHC staining of human liver using 66398-1-Ig (same clone as 66398-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66398-1-Ig (VTN Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Vitronectin/S-protein Monoclonal antibody (66398-1-Ig)

    IHC staining of human liver using 66398-1-Ig (same clone as 66398-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66398-1-Ig (VTN Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Vitronectin/S-protein Monoclonal antibody (66398-1-Ig)

    IF Staining of NIH/3T3 using 66398-1-Ig (same clone as 66398-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using VTN antibody (66398-1-Ig, Clone: 1D2C8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.

Vitronectin/S-protein Monoclonal antibody, PBS Only

Vitronectin/S-protein Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66398-1-PBS
Clone No.1D2C8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

1D2C8, Serum-spreading factor, Somatomedin-B, S-protein, V75

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66398-1-PBS targets Vitronectin/S-protein in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, pig, rabbit samples.

Tested Reactivity human, mouse, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag8443

Product name: Recombinant human VTN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 126-478 aa of BC005046

Sequence: EVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGVLDPDYPRNISDGFDGIPDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEECEGSSLSAVFEHFAMMQRDSWEDIFELLFWGRTSAGTRQPQFISRDWHGVPGQVDAAMAGRIYISGMAPRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQNSRRPSRAMWLSLFSSEESNLGANNYDDYRMDWLVPATCEPIQSVFFFSGDKYYRVNLRTRRVDTVDPPYPRSIAQYWLGCPAPGHL

相同性解析による交差性が予測される生物種
Full Name vitronectin
Calculated molecular weight 478 aa, 54 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC005046
Gene Symbol VTN
Gene ID (NCBI) 7448
RRIDAB_2881772
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP04004
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Vitronectin is a glycoprotein present in blood and in the extracellular matrix (PMID: 10399314). Vitronectin is a multifunctional glycoprotein that mediates cell-to-substrate adhesion, inhibits the cytolytic action of the terminal complement cascade in vitro and binds to several serine protease inhibitors of the serpin family (PMID: 1372588).

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