Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 15833-1-AP
rat plasma was subjected to SDS PAGE followed by western blot with 15833-1-AP (Vitronectin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 15833-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15833-1-AP (Vitronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 15833-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 15833-1-AP (Vitronectin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 15833-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15833-1-AP (Vitronectin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 15833-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15833-1-AP (Vitronectin antibody at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 15833-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15833-1-AP (Vitronectin antibody at dilution of 1:200 (under 40x lens).
IHC staining of human hepatocirrhosis using 15833-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15833-1-AP (Vitronectin antibody at dilution of 1:50 (under 10x lens).
IHC staining of human hepatocirrhosis using 15833-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15833-1-AP (Vitronectin antibody at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 15833-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15833-1-AP (Vitronectin antibody at dilution of 1:200 (under 10x lens).
IHC staining of human testis using 15833-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15833-1-AP (Vitronectin antibody at dilution of 1:200 (under 40x lens).
IF Staining of NIH/3T3 using 15833-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 15833-1-AP (Vitronectin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Vitronectin/S-protein Polyclonal antibody

Vitronectin/S-protein Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15833-1-AP

Publications(32)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Serum-spreading factor, Somatomedin-B, S-protein, V75, Vitronectin

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (32)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	rat plasma, human plasma, human testis tissue, NIH/3T3 cells
Positive IHC detected in	human liver tissue, human hepatocirrhosis tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 21 publications below
IHC	See 7 publications below
IF	See 9 publications below
IP	See 1 publications below

Product Information

15833-1-AP targets Vitronectin/S-protein in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, plasmodium falciparum
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8443 Product name: Recombinant human VTN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 126-478 aa of BC005046 Sequence: EVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGVLDPDYPRNISDGFDGIPDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEECEGSSLSAVFEHFAMMQRDSWEDIFELLFWGRTSAGTRQPQFISRDWHGVPGQVDAAMAGRIYISGMAPRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQNSRRPSRAMWLSLFSSEESNLGANNYDDYRMDWLVPATCEPIQSVFFFSGDKYYRVNLRTRRVDTVDPPYPRSIAQYWLGCPAPGHL 相同性解析による交差性が予測される生物種
Full Name	vitronectin
Calculated molecular weight	478 aa, 54 kDa
Observed molecular weight	70-75 kDa, 65 kDa
GenBank accession number	BC005046
Gene Symbol	VTN
Gene ID (NCBI)	7448
RRID	AB_2216309
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P04004
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Vitronectin is a glycoprotein present in blood and in the extracellular matrix (PMID: 10399314). Vitronectin is a multifunctional glycoprotein that mediates cell-to-substrate adhesion, inhibits the cytolytic action of the terminal complement cascade in vitro and binds to several serine protease inhibitors of the serpin family (PMID: 1372588).

Protocols

Product Specific Protocols
IF protocol for Vitronectin/S-protein antibody 15833-1-AP	Download protocol
IHC protocol for Vitronectin/S-protein antibody 15833-1-AP	Download protocol
WB protocol for Vitronectin/S-protein antibody 15833-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Cell Stem Cell Region-specific cellular and molecular basis of liver regeneration after acute pericentral injury Authors - Shuyong Wang View Article
human, mouse	WB,IP,IHC,IF	Cardiovasc Diabetol Targeting TFAM K76 acetylation attenuates mitochondrial dysfunction and kidney injury in diabetic kidney disease. Authors - Tingting Fu View Article
rat		Acta Biomater Intervertebral disc regeneration with an adipose mesenchymal stem cell-derived tissue-engineered construct in a rat nucleotomy model. Authors - Hiroyuki Ishiguro View Article
rat	IHC	Int J Biol Macromol Multi-omics analysis reveals the pre-protective mechanism of Dendrobium flexicaule polysaccharide against alcohol-induced gastric mucosal injury Authors - Fei Wang View Article
		Cell Mol Gastroenterol Hepatol S-Adenosylmethionine Inhibits Plasminogen-Activating Inhibitor-1 and Protects Male Mice from FOLFOX-Induced Liver Injury Authors - Alexandra Gangi View Article
human	IF	Aging Cell Decellularized Aged Bruch's Membrane Confers Unique Biochemical Cues to Retinal Pigment Epithelium for In Vitro Modeling of Age-Related Macular Degeneration. Authors - Blanca Molins View Article

Validation Data Gallery

WB analysis using 15833-1-AP

WB analysis using 15833-1-AP

WB analysis of human testis using 15833-1-AP

WB analysis of NIH/3T3 using 15833-1-AP

IHC staining of human liver using 15833-1-AP

IHC staining of human liver using 15833-1-AP

IHC staining of human hepatocirrhosis using 15833-1-AP

IHC staining of human hepatocirrhosis using 15833-1-AP

IHC staining of human testis using 15833-1-AP

IHC staining of human testis using 15833-1-AP

IF Staining of NIH/3T3 using 15833-1-AP

Vitronectin/S-protein Polyclonal antibody

Vitronectin/S-protein Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Region-specific cellular and molecular basis of liver regeneration after acute pericentral injury

Targeting TFAM K76 acetylation attenuates mitochondrial dysfunction and kidney injury in diabetic kidney disease.

Intervertebral disc regeneration with an adipose mesenchymal stem cell-derived tissue-engineered construct in a rat nucleotomy model.

Multi-omics analysis reveals the pre-protective mechanism of Dendrobium flexicaule polysaccharide against alcohol-induced gastric mucosal injury

S-Adenosylmethionine Inhibits Plasminogen-Activating Inhibitor-1 and Protects Male Mice from FOLFOX-Induced Liver Injury

Decellularized Aged Bruch's Membrane Confers Unique Biochemical Cues to Retinal Pigment Epithelium for In Vitro Modeling of Age-Related Macular Degeneration.

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