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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse eye tissue using VSX2 Polyclonal antibody (25825-1-AP)

    WB analysis of mouse eye using 25825-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 25825-1-AP (VSX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human retinoblastoma tissue using VSX2 Polyclonal antibody (25825-1-AP)

    IHC staining of human retinoblastoma using 25825-1-AP

    Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 25825-1-AP (VSX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human retinoblastoma tissue using VSX2 Polyclonal antibody (25825-1-AP)

    IHC staining of human retinoblastoma using 25825-1-AP

    Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 25825-1-AP (VSX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using VSX2 Polyclonal antibody (25825-1-AP)

    IHC staining of human lung cancer using 25825-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25825-1-AP (VSX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using VSX2 Polyclonal antibody (25825-1-AP)

    IHC staining of human lung cancer using 25825-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25825-1-AP (VSX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using VSX2 Polyclonal antibody (25825-1-AP)

    IF Staining of mouse eye using 25825-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using VSX2 antibody (25825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using VSX2 Polyclonal antibody (25825-1-AP)

    IF Staining of mouse eye using 25825-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse eye tissue using VSX2 antibody (25825-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

VSX2 Polyclonal antibody

VSX2 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA
Cat No. 25825-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Synonyms

Ceh-10 homeodomain-containing homolog, CHX10, Homeobox protein CHX10, HOX10, MCOP2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse eye tissue
Positive IHC detected inhuman retinoblastoma tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue
Positive IF-Fro detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 2 publications below

Product Information

25825-1-AP targets VSX2 in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VSX2 fusion protein Ag23035 相同性解析による交差性が予測される生物種
Full Name visual system homeobox 2
Calculated molecular weight 361 aa, 39 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC128153
Gene Symbol VSX2
Gene ID (NCBI) 338917
RRIDAB_2880257
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP58304
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for VSX2 antibody 25825-1-APDownload protocol
IHC protocol for VSX2 antibody 25825-1-APDownload protocol
IF protocol for VSX2 antibody 25825-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Stem Cell

Human brain organoids assemble functionally integrated bilateral optic vesicles.

Authors - Elke Gabriel
View Article
humanIF

Nat Protoc

Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia

Authors - Elke Gabriel
View Article