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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VSTM1 Polyclonal antibody (24382-1-AP)

    WB analysis using 24382-1-AP (same clone as 24382-1-PBS)

    human peripheral blood leukocytes were subjected to SDS PAGE followed by western blot with 24382-1-AP (VSTM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24382-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using VSTM1 Polyclonal antibody (24382-1-AP)

    WB analysis of HL-60 using 24382-1-AP (same clone as 24382-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 24382-1-AP (VSTM1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24382-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VSTM1 Polyclonal antibody (24382-1-AP)

    IHC staining of human tonsillitis using 24382-1-AP (same clone as 24382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24382-1-AP (VSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24382-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VSTM1 Polyclonal antibody (24382-1-AP)

    IHC staining of human tonsillitis using 24382-1-AP (same clone as 24382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24382-1-AP (VSTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24382-1-PBS in a different storage buffer formulation.

VSTM1 Polyclonal antibody, PBS Only

VSTM1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24382-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24382-1-PBS targets VSTM1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15799

Product name: Recombinant human VSTM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 14-140 aa of BC100942

Sequence: CLGYEDEKKNEKPPKPSLHAWPSSVVEAESNVTLKCQAHSQNVTFVLRKVNDSGYKQEQSSAENEAEFPFTDLKPKDAGRYFCAYKTTASHEWSESSEHLQLVVTDKHDELEAPSMKTDTRTIFVAI

相同性解析による交差性が予測される生物種
Full Name V-set and transmembrane domain containing 1
Calculated molecular weight 236 aa, 26 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC100942
Gene Symbol VSTM1
Gene ID (NCBI) 284415
RRIDAB_2879516
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UX27
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

VSTM1 (V-set and transmembrane domain-containing protein 1) has two main splicing isoform, VSTM1-v1 and VSTM1-v2. VSTM1-v1 is a type I membrane molecule, mainly expressed on human peripheral blood granulocytes and monocytes. VSTM1-v2 is a classical secretory glycoprotein mainly expressed in immune tissues, and can promote the differentiation and activation of Th17 cells. (PMID: 22960280; 23909423)

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