Validation Data Gallery
Tested Applications
| Positive WB detected in | TT cells, U-251 cells, U-87 MG cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27829-1-AP targets VSIG4 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27214 Product name: Recombinant human VSIG4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 305-399 aa of BC010525 Sequence: MLCRKTSQQEHVYEAARAHAREANDSGETMRVAIFASGCSSDEPTSQNLGNNYSDEPCIGQEYQIIAQINGNYARLLDTVPLDYEFLATEGKSVC 相同性解析による交差性が予測される生物種 |
| Full Name | V-set and immunoglobulin domain containing 4 |
| Calculated molecular weight | 399 aa, 44 kDa |
| Observed molecular weight | 44 kDa |
| GenBank accession number | BC010525 |
| Gene Symbol | VSIG4 |
| Gene ID (NCBI) | 11326 |
| RRID | AB_3742260 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y279 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
V-set and immunoglobulin domain-containing 4 (VSIG4) is a complement receptor of the immunoglobulin superfamily (CRIg) that is specifically expressed in resting tissue-resident macrophages . Notably, CRIg is expressed in monocyte-derived macrophages but not in human and mouse monocytes. VSIG4 can suppress T cell proliferation and promote Foxp3+ regulatory T cell (Tregs) differentiation by binding an unidentified receptor on T cells . VSIG4 has been implicated in a variety of diseases, particularly those involving immune dysregulation and inflammation, such as non-alcoholic steatohepatitis, rheumatoid arthritis, and obesity-related metabolic disorders . Recently, VSIG4+ tumor-associated macrophages (TAMs) have been identified as a specific subset that infiltrates aggressive cancers, including anaplastic thyroid cancer (ATC). (PMID: 37153746,PMID: 39920772)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VSIG4 antibody 27829-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |