Validation Data Gallery
Tested Applications
| Positive WB detected in | 786-O cells, rat liver tissue, ACHN cells, Calu-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33200-1-AP targets VSIG10 in WB, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37432 Product name: Recombinant human FLJ20674 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 31-250 aa of BC034471 Sequence: VVIGEVHENVTLHCGNISGLRGQVTWYRNNSEPVFLLSSNSSLRPAEPRFSLVDATSLHIESLSLGDEGIYTCQEILNVTQWFQVWLQVASGPYQIEVHIVATGTLPNGTLYAARGSQVDFSCNSSSRPPPVVEWWFQALNSSSESFGHNLTVNFFSLLLISPNLQGNYTCLALNQLSKRHRKVTTELLVYYPPPSAPQCWAQMASGSFMLQLTCRWDGG 相同性解析による交差性が予測される生物種 |
| Full Name | hypothetical protein FLJ20674 |
| Calculated molecular weight | 60 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC034471 |
| Gene Symbol | FLJ20674 |
| Gene ID (NCBI) | 54621 |
| RRID | AB_3742892 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8N0Z9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VSIG10 (V-set and immunoglobulin domain containing 10) is a protein-coding gene located on human chromosome 12q24.23 that encodes a transmembrane protein belonging to the immunoglobulin superfamily. VSIG10 is a protein whose expression is altered in response to SARS-CoV-2 infection, making it a novel candidate biomarker for COVID-19.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VSIG10 antibody 33200-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |