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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VRK3 Polyclonal antibody

VRK3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15608-1-AP

Synonyms

vaccinia related kinase 3, VRK3

Validation Data Gallery

Applications:
  • WB analysis using 15608-1-AP

    WB analysis using 15608-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15608-1-AP (VRK3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 15608-1-AP

    WB analysis of HeLa using 15608-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15608-1-AP (VRK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 15608-1-AP

    WB analysis of HeLa using 15608-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15608-1-AP (VRK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 15608-1-AP

    IHC staining of human breast cancer using 15608-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15608-1-AP (VRK3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 15608-1-AP

    IHC staining of human breast cancer using 15608-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15608-1-AP (VRK3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHL-60 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

15608-1-AP targets VRK3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VRK3 fusion protein Ag7993 相同性解析による交差性が予測される生物種
Full Name vaccinia related kinase 3
Calculated molecular weight 53 kDa
Observed molecular weight 50-53 kDa
GenBank accession numberBC023556
Gene symbol VRK3
Gene ID (NCBI) 51231
RRIDAB_10863120
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for VRK3 antibody 15608-1-APDownload protocol
IHC protocol for VRK3 antibody 15608-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols