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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using VPS54 Monoclonal antibody (67404-1-Ig)

    WB analysis of SH-SY5Y using 67404-1-Ig (same clone as 67404-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67404-1-Ig (VPS54 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using VPS54 Monoclonal antibody (67404-1-Ig)

    WB analysis of pig brain using 67404-1-Ig (same clone as 67404-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67404-1-Ig (VPS54 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VPS54 Monoclonal antibody (67404-1-Ig)

    IHC staining of human liver cancer using 67404-1-Ig (same clone as 67404-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67404-1-Ig (VPS54 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VPS54 Monoclonal antibody (67404-1-Ig)

    IHC staining of human liver cancer using 67404-1-Ig (same clone as 67404-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67404-1-Ig (VPS54 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51221-2

    Cytometric bead array standard curve of MP51221-2

    Cytometric bead array standard curve of MP51221-2, VPS54 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67404-2-PBS. Detection antibody: 67404-1-PBS. Standard:Ag4123. Range: 0.195-25 ng/mL.

  • Cytometric bead array standard curve of MP51221-3

    Cytometric bead array standard curve of MP51221-3

    Cytometric bead array standard curve of MP51221-3, VPS54 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67404-3-PBS. Detection antibody: 67404-1-PBS. Standard:Ag4123. Range: 0.195-12.5 ng/mL.

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VPS54 Monoclonal antibody, PBS Only (Detector)

VPS54 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 67404-1-PBS
Clone No.3D12D1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

Vacuolar protein sorting-associated protein 54, Tumor antigen SLP-8p, Tumor antigen HOM-HCC-8, Hepatocellular carcinoma protein 8, HCC8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67404-1-PBS targets VPS54 as part of a matched antibody pair:

MP51221-2: 67404-2-PBS capture and 67404-1-PBS detection (validated in Cytometric bead array)

MP51221-3: 67404-3-PBS capture and 67404-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VPS54 fusion protein Ag4123 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 54 homolog (S. cerevisiae)
Calculated molecular weight 977 aa, 111 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC030275
Gene Symbol VPS54
Gene ID (NCBI) 51542
RRIDAB_2882645
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9P1Q0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

VPS54 is a part of the Golgi-associated retrograde protein (GARP) complex protein required for tethering and fusion of endosome-derived transport vesicles to the trans-Golgi network. It may particpate in retrograde transport from early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD.