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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using VPS54 Polyclonal antibody (13327-1-AP)

    WB analysis of human brain using 13327-1-AP (same clone as 13327-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 13327-1-AP (VPS54 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13327-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using VPS54 Polyclonal antibody (13327-1-AP)

    WB analysis of human brain using 13327-1-AP (same clone as 13327-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 13327-1-AP (VPS54 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13327-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using VPS54 Polyclonal antibody (13327-1-AP)

    WB analysis of mouse brain using 13327-1-AP (same clone as 13327-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13327-1-AP (VPS54 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13327-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using VPS54 Polyclonal antibody (13327-1-AP)

    WB analysis of human brain using 13327-1-AP (same clone as 13327-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 13327-1-AP (VPS54 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13327-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using VPS54 Polyclonal antibody (13327-1-AP)

    IP experiment of mouse brain using 13327-1-AP (same clone as 13327-1-PBS)

    IP result of anti-VPS54 (IP:13327-1-AP, 4ug; Detection:13327-1-AP 1:300) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 13327-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

VPS54 Polyclonal antibody, PBS Only

VPS54 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 13327-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Vacuolar protein sorting-associated protein 54, Tumor antigen SLP-8p, Tumor antigen HOM-HCC-8, Hepatocellular carcinoma protein 8, HCC8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13327-1-PBS targets VPS54 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS54 fusion protein Ag4123 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 54 homolog (S. cerevisiae)
Calculated molecular weight 977 aa, 111 kDa
Observed molecular weight 111 kDa
GenBank accession numberBC030275
Gene Symbol VPS54
Gene ID (NCBI) 51542
RRIDAB_2304414
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P1Q0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

VPS54 is a part of the Golgi-associated retrograde protein (GARP) complex protein required for tethering and fusion of endosome-derived transport vesicles to the trans-Golgi network. It may particpate in retrograde transport from early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD.