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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using VPS54 Polyclonal antibody (13327-1-AP)

    WB analysis of human brain using 13327-1-AP (same clone as 13327-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 13327-1-AP (VPS54 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13327-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using VPS54 Polyclonal antibody (13327-1-AP)

    WB analysis using 13327-1-AP (same clone as 13327-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13327-1-AP (VPS54 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13327-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using VPS54 Polyclonal antibody (13327-1-AP)

    WB analysis of mouse brain using 13327-1-AP (same clone as 13327-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13327-1-AP (VPS54 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13327-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using VPS54 Polyclonal antibody (13327-1-AP)

    WB analysis of human brain using 13327-1-AP (same clone as 13327-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 13327-1-AP (VPS54 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13327-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using VPS54 Polyclonal antibody (13327-1-AP)

    IP experiment of mouse brain using 13327-1-AP (same clone as 13327-1-PBS)

    IP result of anti-VPS54 (IP:13327-1-AP, 4ug; Detection:13327-1-AP 1:300) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 13327-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

VPS54 Polyclonal antibody, PBS Only

VPS54 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 13327-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

HCC8, Hepatocellular carcinoma protein 8, Tumor antigen HOM-HCC-8, Tumor antigen SLP-8p, Vacuolar protein sorting-associated protein 54

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13327-1-PBS targets VPS54 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4123

Product name: Recombinant human VPS54 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 746-965 aa of BC030275

Sequence: CVDNIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQYSMLRHFDHITKDYHDHIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSACFRNICKQMTKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLKDLDLNMAEIWEQKR

相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 54 homolog (S. cerevisiae)
Calculated molecular weight 977 aa, 111 kDa
Observed molecular weight 90-93kDa, 111 kDa
GenBank accession numberBC030275
Gene Symbol VPS54
Gene ID (NCBI) 51542
RRIDAB_2304414
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P1Q0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

VPS54 is a part of the Golgi-associated retrograde protein (GARP) complex protein required for tethering and fusion of endosome-derived transport vesicles to the trans-Golgi network. It may particpate in retrograde transport from early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD.

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