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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VPS4A/B Polyclonal antibody (17673-1-AP)

    WB analysis using 17673-1-AP (same clone as 17673-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4A/B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17673-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skin tissue using VPS4A/B Polyclonal antibody (17673-1-AP)

    WB analysis of mouse skin using 17673-1-AP (same clone as 17673-1-PBS)

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17673-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using VPS4A/B Polyclonal antibody (17673-1-AP)

    IP experiment of HeLa using 17673-1-AP (same clone as 17673-1-PBS)

    IP result of anti-VPS4A/B (IP:17673-1-AP, 4ug; Detection:17673-1-AP 1:3000) with HeLa cells lysate 1360 ug. This data was developed using the same antibody clone with 17673-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using VPS4A/B Polyclonal antibody (17673-1-AP)

    FC experiment of A431 using 17673-1-AP (same clone as 17673-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug VPS4A/B Polyclonal antibody (17673-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 17673-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

VPS4A/B Polyclonal antibody, PBS Only

VPS4A/B Polyclonal Antibody for WB, FC (Intra), IP, Indirect ELISA
Cat No. 17673-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), IP, Indirect ELISA

Synonyms

VPS4B, Vacuolar protein sorting-associated protein 4B, SKD1, Protein SKD1, MIG1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17673-1-PBS targets VPS4A/B in WB, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11610

Product name: Recombinant human VPS4B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-444 aa of BC039574

Sequence: MSSTSPNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAEKLKEYLKNKEKKAQKPVKEGQPSPADEKGNDSDGEGESDDPEKKKLQNQLQGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTTQNSLTEADFRELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMSDMLRSLSNTKPTVNEHDLLKLKKFTEDFGQEG

相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 4 homolog B (S. cerevisiae)
Calculated molecular weight 444 aa, 49 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC039574
Gene Symbol VPS4B
Gene ID (NCBI) 9525
RRIDAB_2215504
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75351
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. Human VPS4 has two orthologous proteins named VPS4A and VPS4B. This antibody can recognize both VPS4A and VPS4B due to their high homology.

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