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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VPS4A Polyclonal antibody (14272-1-AP)

    WB analysis using 14272-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using VPS4A Polyclonal antibody (14272-1-AP)

    WB analysis of U-937 using 14272-1-AP

    WB result of VPS4A antibody (14272-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS4A transfected U-937 cells.

  • Western Blot (WB) analysis of various lysates using VPS4A Polyclonal antibody (14272-1-AP)

    WB analysis using 14272-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using VPS4A Polyclonal antibody (14272-1-AP)

    WB analysis of SMMC-7721 using 14272-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using VPS4A Polyclonal antibody (14272-1-AP)

    WB analysis of SMMC-7721 using 14272-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using VPS4A Polyclonal antibody (14272-1-AP)

    WB analysis of HepG2 using 14272-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using VPS4A Polyclonal antibody (14272-1-AP)

    IHC staining of human kidney using 14272-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14272-1-AP (VPS4A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using VPS4A Polyclonal antibody (14272-1-AP)

    IHC staining of human kidney using 14272-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14272-1-AP (VPS4A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using VPS4A Polyclonal antibody (14272-1-AP)

    IHC staining of human stomach cancer using 14272-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14272-1-AP (VPS4A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using VPS4A Polyclonal antibody (14272-1-AP)

    IHC staining of mouse pancreas using 14272-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14272-1-AP (VPS4A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using VPS4A Polyclonal antibody (14272-1-AP)

    IHC staining of rat kidney using 14272-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 14272-1-AP (VPS4A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat lung tissue using VPS4A Polyclonal antibody (14272-1-AP)

    IHC staining of rat lung using 14272-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 14272-1-AP (VPS4A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using VPS4A Polyclonal antibody (14272-1-AP)

    IHC staining of rat stomach using 14272-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 14272-1-AP (VPS4A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using VPS4A Polyclonal antibody (14272-1-AP)

    IHC staining of mouse kidney using 14272-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14272-1-AP (VPS4A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using VPS4A Polyclonal antibody (14272-1-AP)

    IF Staining of U2OS using 14272-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using VPS4A antibody (14272-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

VPS4A Polyclonal antibody

VPS4A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14272-1-AP
Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:3.6.4.6, hVPS4, Protein SKD2, SKD1, SKD2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU-937 cells, SMMC-7721 cells, HepG2 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman kidney tissue, rat stomach tissue, mouse pancreas tissue, rat kidney tissue, human stomach cancer tissue, rat lung tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14272-1-AP targets VPS4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5592

Product name: Recombinant human VPS4A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 86-437 aa of BC047932

Sequence: KENQSEGKGSDSDSEGDNPEKKKLQEQLMGAVVMEKPNIRWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVKNLFELARQHKPSIIFIDEVDSLCGSRNENESEAARRIKTEFLVQMQGVGNNNDGTLVLGATNIPWVLDSAIRRRFEKRIYIPLPEEAARAQMFRLHLGSTPHNLTDANIHELARKTEGYSGADISIIVRDSLMQPVRKVQSATHFKKVCGPSRTNPSMMIDDLLTPCSPGDPGAMEMTWMDVPGDKLLEPVVCMSDMLRSLATTRPTVNADDLLKVKKFSEDFGQES

相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 4 homolog A (S. cerevisiae)
Calculated molecular weight 49 kDa
Observed molecular weight 48-55 kDa
GenBank accession numberBC047932
Gene Symbol VPS4A
Gene ID (NCBI) 27183
RRIDAB_2878039
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UN37
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vacuolar Protein Sorting 4A (VPS4A) is one of two human homologs of the yeast protein Vps4 and member of the AAA protein family (ATPases associated with diverse cellular activities).

Protocols

Product Specific Protocols
IF protocol for VPS4A antibody 14272-1-APDownload protocol
IHC protocol for VPS4A antibody 14272-1-APDownload protocol
WB protocol for VPS4A antibody 14272-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB

Nat Commun

Negative feedback regulation of STING signaling by TAX1BP1-directed Golgiphagy.

Authors - Sujit Suklabaidya
View Article
mouseWB,IF

Mol Cell

VPS4A is the selective receptor for lipophagy in mice and humans

Authors - Debajyoti Das
View Article
humanWB

Theranostics

Endosome-microautophagy targeting chimera (eMIATAC) for targeted proteins degradation and enhance CAR-T cell anti-tumor therapy

Authors - Kunjian Lei
View Article
humanWB

Proc Natl Acad Sci U S A

Endosome transcriptomics reveal trafficking of Cajal bodies into multivesicular bodies.

Authors - Jasleen Singh
View Article

Proc Natl Acad Sci U S A

Negative interplay between HIV-1 Gag and amyloid precursor protein centers around competition for VPS4A and TSG101

Authors - Feng Gu
View Article

Cell Rep

MITD1 restricts HIV-1 release by targeting ESCRT-III proteins.

Authors - Jim Zoladek
View Article
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