Validation Data Gallery
Tested Applications
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human heart tissue, human small intestine tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| IF | See 4 publications below |
| IP | See 2 publications below |
Product Information
16219-1-AP targets VPS39 in IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9267 Product name: Recombinant human VPS39 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 437-786 aa of BC015817 Sequence: ERTVQYLQHLGTENLHLIFSYSVWVLRDFPEDGLKIFTEDLPEVESLPRDRVLGFLIENFKGLAIPYLEHIIHVWEETGSRFHNCLIQLYCEKVQGLMKEYLLSFPAGKTPVPAGEEEGELGEYRQKLLMFLEISSYYDPGRLICDFPFDGLLEERALLLGRMGKHEQALFIYVHILKDTRMAEEYCHKHYDRNKDGNKDVYLSLLRMYLSPPSIHCLGPIKLELLEPKANLQAALQVLELHHSKLDTTKALNLLPANTQINDIRIFLEKVLEENAQKKRFNQVLKNLLHAEFLRVQEERILHQQVKCIITEEKVCMVCKKKIGNSAFARYPNGVVVHYFCSKEVNPADT 相同性解析による交差性が予測される生物種 |
| Full Name | vacuolar protein sorting 39 homolog (S. cerevisiae) |
| Calculated molecular weight | 886 aa, 102 kDa |
| Observed molecular weight | 102 kDa |
| GenBank accession number | BC015817 |
| Gene Symbol | VPS39 |
| Gene ID (NCBI) | 23339 |
| RRID | AB_11043179 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96JC1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VPS39, also named as KIAA0770, TLP, or VAM6, is a 886 amino acid protein, which contains one CNH domain and one CHCR repeat. VPS39 belongs to the VAM6/VPS39 family and localizes in the cytoplasm. VPS39 is widely expressed, with highest levels in heart, skeletal muscle, kidney, pancreas, brain, placenta and spleen. VPS39 may play a role in clustering and fusion of late endosomes and lysosomes. VPS39 modulates the transforming growth factor-beta response by coupling the transforming growth factor-beta receptor complex to the Smad pathway.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for VPS39 antibody 16219-1-AP | Download protocol |
| IP protocol for VPS39 antibody 16219-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy | ||
Cell Host Microbe Global mapping of Salmonella enterica-host protein-protein interactions during infection. | ||
Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes | ||
Autophagy Atractylenolide I inhibits angiogenesis and reverses sunitinib resistance in clear cell renal cell carcinoma through ATP6V0D2-mediated autophagic degradation of EPAS1/HIF2α | ||
Dev Cell ORF3a of SARS-CoV-2 promotes lysosomal exocytosis-mediated viral egress
| ||
Curr Biol Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy. |