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Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using VPS36 Polyclonal antibody (14262-1-AP)

    WB analysis of HL-60 using 14262-1-AP (same clone as 14262-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 14262-1-AP (VPS36 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14262-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using VPS36 Polyclonal antibody (14262-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 14262-1-AP (same clone as 14262-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 14262-1-AP (VPS36 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14262-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using VPS36 Polyclonal antibody (14262-1-AP)

    IHC staining of human pancreas cancer using 14262-1-AP (same clone as 14262-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14262-1-AP (VPS36 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14262-1-PBS in a different storage buffer formulation.

VPS36 Polyclonal antibody, PBS Only

VPS36 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14262-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

ELL-associated protein of 45 kDa, EAP45, CGI-145, CGI145, CGI 145

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14262-1-PBS targets VPS36 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5549

Product name: Recombinant human VPS36 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-386 aa of BC050439

Sequence: MDRFVWTSGLLEINETLVIQQRGVRIYDGEEKIKFDAGTLLLSTHRLIWRDQKNHECCMAILLSQIVFIEEQAAGIGKSAKIVVHLHPAPPNKEPGPFQSSKNSYIKLSFKEHGQIEFYRRLSEEMTQRRWENMPVSQSLQTNRGPQPGRIRAVGIVGIERKLEEKRKETDKNISEAFEDLSKLMIKAKEMVELSKSIANKIKDKQGDITEDETIRFKSYLLSMGIANPVTRETYGSGTQYHMQLAKQLAGILQVPLEERGGIMSLTEVYCLVNRARGMELLSPEDLVNACKMLEALKLPLRLRVFDSGVMVIELQSHKEEEMVASALETVSEKGSLTSEEFAKLVGMSVLLAKERLLLAEKMGHLCRDDSVEGLRFYPNLFMTQS

相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 36 homolog (S. cerevisiae)
Calculated molecular weight 44 kDa
Observed molecular weight 43 kDa, 65 kDa
GenBank accession numberBC050439
Gene Symbol VPS36
Gene ID (NCBI) 51028
RRIDAB_2878036
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86VN1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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