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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VPS25 Monoclonal antibody (68197-1-Ig)

    WB analysis using 68197-1-Ig (same clone as 68197-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68197-1-Ig (VPS25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68197-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit kidney tissue using VPS25 Monoclonal antibody (68197-1-Ig)

    WB analysis of rabbit kidney using 68197-1-Ig (same clone as 68197-1-PBS)

    rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68197-1-Ig (VPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68197-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using VPS25 Monoclonal antibody (68197-1-Ig)

    WB analysis of HeLa using 68197-1-Ig (same clone as 68197-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68197-1-Ig (VPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68197-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using VPS25 Monoclonal antibody (68197-1-Ig)

    IF Staining of HepG2 using 68197-1-Ig (same clone as 68197-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VPS25 antibody (68197-1-Ig, Clone: 3B4C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68197-1-PBS in a different storage buffer formulation.

VPS25 Monoclonal antibody, PBS Only

VPS25 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68197-1-PBS
Clone No.3B4C3

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

DERP9, EAP20, ESCRT II complex subunit VPS25, FAP20, hVps25, VPS25

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68197-1-PBS targets VPS25 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Rabbit samples.

Tested Reactivity Human, Rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag8243

Product name: Recombinant human VPS25 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-176 aa of BC006282

Sequence: MAMSFEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVKLQRKLPVESIQIVLEELRKKGNLEWLDKSKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDEEFHGLDEATLLRALQALQQEHKAEIITVSDGRGVKFF

相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 25 homolog (S. cerevisiae)
Calculated molecular weight 176 aa, 21 kDa
Observed molecular weight19 kDa
GenBank accession numberBC006282
Gene Symbol VPS25
Gene ID (NCBI) 84313
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9BRG1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

VPS25 is a component of the endosome-associated complex ESCRT-II (Endosomal Sorting Complexes Required for Transport protein II). ESCRT-II complex, composed of VPS25, VPS36, and SNF8, functions in sorting of ubiquitinated membrane proteins during endocytosis. ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. VPS25 is highly conserved. Human VPS25 gene maps to chromosome 17q21.31 and is expressed in a wide range of tissues (PMID: 16889659).

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