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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

VPS24 Polyclonal antibody

VPS24 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15472-1-AP

Synonyms

CGI 149, CHMP3, Chromatin modifying protein 3, hVps24, NEDF, VPS24

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 15472-1-AP

    WB analysis of HEK-293 using 15472-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15472-1-AP (VPS24 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of human kidney using 15472-1-AP

    WB analysis of human kidney using 15472-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15472-1-AP (VPS24 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 15472-1-AP

    IP experiment of HEK-293 using 15472-1-AP

    IP result of anti-VPS24 (IP:15472-1-AP, 4ug; Detection:15472-1-AP 1:500) with HEK-293 cells lysate 1480ug.

  • IHC staining of human heart using 15472-1-AP

    IHC staining of human heart using 15472-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15472-1-AP (VPS24 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human heart using 15472-1-AP

    IHC staining of human heart using 15472-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15472-1-AP (VPS24 antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HepG2 using 15472-1-AP

    IF Staining of HepG2 using 15472-1-AP

    Immunofluorescent analysis of HepG2 cells, using VPS24 antibody 15472-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IF Staining of HepG2 using 15472-1-AP

    IF Staining of HepG2 using 15472-1-AP

    Immunofluorescent analysis of HepG2 cells using 15472-1-AP (VPS24 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, human kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15472-1-AP targets VPS24 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS24 fusion protein Ag7776 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 24 homolog (S. cerevisiae)
Calculated molecular weight 25 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC004419
Gene symbol VPS24
Gene ID (NCBI) 51652
RRIDAB_2217102
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for VPS24 antibody 15472-1-APDownload protocol
IHC protocol for VPS24 antibody 15472-1-APDownload protocol
IF protocol for VPS24 antibody 15472-1-APDownload protocol
IP protocol for VPS24 antibody 15472-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Cell Biol

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Authors - Vincent Mercier
View Article
humanWB

J Cell Biol

ALIX- and ESCRT-III-dependent sorting of tetraspanins to exosomes.

Authors - Jorge Larios
  • KD Validated
View Article
humanWB,IHC

Int J Oncol

CHMP3 promotes the progression of hepatocellular carcinoma by inhibiting caspase‑1‑dependent pyroptosis

Authors - Yuting Zheng
  • KD Validated
View Article