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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using VPS18 Polyclonal antibody (10901-1-AP)

    WB analysis of mouse brain using 10901-1-AP (same clone as 10901-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10901-1-AP (VPS18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using VPS18 Polyclonal antibody (10901-1-AP)

    IP experiment of HeLa using 10901-1-AP (same clone as 10901-1-PBS)

    IP result of anti-VPS18 (IP:10901-1-AP, 4ug; Detection:10901-1-AP 1:300) with HeLa cells lysate 920ug. This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using VPS18 Polyclonal antibody (10901-1-AP)

    IHC staining of human lung cancer using 10901-1-AP (same clone as 10901-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10901-1-AP (VPS18 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using VPS18 Polyclonal antibody (10901-1-AP)

    IHC staining of human lung cancer using 10901-1-AP (same clone as 10901-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10901-1-AP (VPS18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VPS18 Polyclonal antibody (10901-1-AP)

    IHC staining of mouse brain using 10901-1-AP (same clone as 10901-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10901-1-AP (VPS18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VPS18 Polyclonal antibody (10901-1-AP)

    IHC staining of mouse brain using 10901-1-AP (same clone as 10901-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10901-1-AP (VPS18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using VPS18 Polyclonal antibody (10901-1-AP)

    IHC staining of mouse testis using 10901-1-AP (same clone as 10901-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10901-1-AP (VPS18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using VPS18 Polyclonal antibody (10901-1-AP)

    IHC staining of mouse testis using 10901-1-AP (same clone as 10901-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10901-1-AP (VPS18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using VPS18 Polyclonal antibody (10901-1-AP)

    IHC staining of human testis using 10901-1-AP (same clone as 10901-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10901-1-AP (VPS18 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using VPS18 Polyclonal antibody (10901-1-AP)

    IHC staining of human testis using 10901-1-AP (same clone as 10901-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10901-1-AP (VPS18 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using VPS18 Polyclonal antibody (10901-1-AP)

    IF Staining of A549 using 10901-1-AP (same clone as 10901-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using VPS18 antibody (10901-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

VPS18 Polyclonal antibody, PBS Only

VPS18 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10901-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Vacuolar protein sorting-associated protein 18 homolog, KIAA1475, hVPS18

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10901-1-PBS targets VPS18 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1140

Product name: Recombinant human VPS18 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 226-355 aa of BC001513

Sequence: QRLFQFIGRAAEGAEAQGFSGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMMGDGVLYGALDCGRPDSLLSEERVWEYPEGVGPGASPPLAIVLTQFHFLLLLADRVEAVCTLTGQVVL

相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 18 homolog (S. cerevisiae)
Calculated molecular weight 110 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC001513
Gene Symbol VPS18
Gene ID (NCBI) 57617
RRIDAB_2273089
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P253
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Vps18 is a central member of Vps-C complex. Vps18 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

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