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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using VPS15 Polyclonal antibody (17894-1-AP)

    WB analysis of HepG2 using 17894-1-AP (same clone as 17894-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17894-1-AP (VPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17894-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using VPS15 Polyclonal antibody (17894-1-AP)

    WB analysis using 17894-1-AP (same clone as 17894-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17894-1-AP (VPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17894-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VPS15 Polyclonal antibody (17894-1-AP)

    IF Staining of HeLa using 17894-1-AP (same clone as 17894-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VPS15 antibody (17894-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 17894-1-PBS in a different storage buffer formulation.

VPS15 Polyclonal antibody, PBS Only

VPS15 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 17894-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17894-1-PBS targets VPS15 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12221

Product name: Recombinant human PIK3R4 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-330 aa of BC110318

Sequence: MGNQLAGIAPSQILSVESYFSDIHDFEYDKSLGSTRFFKVARAKHREGLVVVKVFAIQDPTLPLTSYKQELEELKIRLNSAQNCLPFQKASEKASEKAAMLFRQYVRDNLYDRISTRPFLNNIEKRWIAFQILTAVDQAHKSGVRHGDIKTENVMVTSWNWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERFVDGGMFATELEYMRDPSTPLVDLNSNQRTRGELKRAMDIFSAGCVIAELFTEGVPLFDLSQLLAYRNGHFFPEQVLNKIEDHSIRELVTQMIHREPDKRLEAEDYLKQQRGNAFPEIFYTFLQPYMAQ

相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, regulatory subunit 4
Calculated molecular weight 1358 aa, 153 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC110318
Gene Symbol PIK3R4
Gene ID (NCBI) 30849
RRIDAB_2163630
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99570
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PIK3R4(phosphoinositide 3-kinase regulatory subunit 4) is also named as PI3-kinase p150 subunit and belongs to the Ser/Thr protein kinase family. It is a regulatory subunit of the PI3K complex and may regulate membrane trafficking late in the endocytic pathway. This protein can be probably autophosphorylated(PMID:19369195).

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