Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse brain tissue, mouse heart tissue |
| Positive IHC detected in | mouse brain tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
Product Information
28618-1-AP targets VPS13A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29532 Product name: Recombinant human VPS13A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2452-2595 aa of NM_033305 Sequence: PVGSRRLKWRCRKSHGEVTQKDDMMMPIDLGEKTIYLVSFFEGLQRIILFTEDPRVFKVTYESEKAELAEQEIAVALQDVGISLVNNYTKQEVAYIGITSSDVVWETKPKKKARWKPMSVKHTEKLEREFKEYTESSPSEDKVI 相同性解析による交差性が予測される生物種 |
| Full Name | vacuolar protein sorting 13 homolog A (S. cerevisiae) |
| Calculated molecular weight | 360 kDa |
| Observed molecular weight | 360 kDa |
| GenBank accession number | NM_033305 |
| Gene Symbol | VPS13A |
| Gene ID (NCBI) | 23230 |
| RRID | AB_2918183 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96RL7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VPS13 (vacuolar protein sorting 13) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human - VPS13A, VPS13B, VPS13C and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for VPS13A antibody 28618-1-AP | Download protocol |
| WB protocol for VPS13A antibody 28618-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS Biol Impaired hematopoiesis and embryonic lethality at midgestation of mice lacking both lipid transfer proteins VPS13A and VPS13C.
| ||
Int J Mol Sci Analysis of Brain, Blood, and Testis Phenotypes Lacking the Vps13a Gene in C57BL/6N Mice | ||
bioRxiv Defect in hematopoiesis and embryonic lethality at midgestation of Vps13a/Vps13c double knockout mice
| ||
Cell Death Discov Insights from lipidomics into the terminal maturation of circulating human reticulocytes |