Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 21921-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21921-1-AP (VMA21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 21921-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 21921-1-AP (VMA21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 21921-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 21921-1-AP (VMA21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 21921-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 21921-1-AP (VMA21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 21921-1-AP
IP result of anti-VMA21 (IP:21921-1-AP, 3ug; Detection:21921-1-AP 1:1000) with MCF-7 cells lysate 4000ug.
IHC staining of mouse brain using 21921-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21921-1-AP (VMA21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 21921-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 21921-1-AP (VMA21 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 21921-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 21921-1-AP (VMA21 antibody) at dilution of 1:50 (under 40x lens).

VMA21 Polyclonal antibody

VMA21 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 21921-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

MEAX, VMA21, XMEA

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, MCF-7 cells, human placenta tissue, mouse thymus tissue, HepG2 cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	mouse brain tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below

Product Information

21921-1-AP targets VMA21 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	VMA21 fusion protein Ag16439 相同性解析による交差性が予測される生物種
Full Name	VMA21 vacuolar H+-ATPase homolog (S. cerevisiae)
Calculated molecular weight	101 aa, 11 kDa
Observed molecular weight	11 kDa
GenBank accession number	BC105694
Gene Symbol	VMA21
Gene ID (NCBI)	203547
RRID	AB_10896457
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q3ZAQ7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for VMA21 antibody 21921-1-AP	Download protocol
IHC protocol for VMA21 antibody 21921-1-AP	Download protocol
IP protocol for VMA21 antibody 21921-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature CFIm25 links alternative polyadenylation to glioblastoma tumour suppression. Authors - Chioniso P Masamha View Article
human	WB	Autophagy Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency Authors - Fangyang Wang View Article
human,mouse	WB	J Transl Med Hsa_circ_0001361 facilitates the progress of lung adenocarcinoma cells via targeting miR-525-5p/VMA21 axis. Authors - Hong-Yu Shen View Article
mouse	WB	Autophagy Aerobic exercise attenuates autophagy-lysosomal flux deficits by ADRB2/β2-adrenergic receptor-mediated V-ATPase assembly factor VMA21 signaling in APP-PSEN1/PS1 mice Authors - Jia-Jun Wu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 21921-1-AP

WB analysis of MCF-7 using 21921-1-AP

WB analysis of human placenta using 21921-1-AP

WB analysis of mouse thymus using 21921-1-AP

IP experiment of MCF-7 using 21921-1-AP

IHC staining of mouse brain using 21921-1-AP

IHC staining of human kidney using 21921-1-AP