Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, MCF-7 cells, human placenta tissue, mouse thymus tissue, HepG2 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | mouse brain tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
21921-1-AP targets VMA21 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16439 Product name: Recombinant human VMA21 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-101 aa of BC105694 Sequence: MERPDKAALNALQPPEFRNESSLASTLKTLLFFTALMITVPIGLYFTTKSYIFEGALGMSNRDSYFYAAIVAVVAVHVVLALFVYVAWNEGSRQWREGKQD 相同性解析による交差性が予測される生物種 |
| Full Name | VMA21 vacuolar H+-ATPase homolog (S. cerevisiae) |
| Calculated molecular weight | 101 aa, 11 kDa |
| Observed molecular weight | 11 kDa |
| GenBank accession number | BC105694 |
| Gene Symbol | VMA21 |
| Gene ID (NCBI) | 203547 |
| RRID | AB_10896457 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q3ZAQ7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for VMA21 antibody 21921-1-AP | Download protocol |
| IP protocol for VMA21 antibody 21921-1-AP | Download protocol |
| WB protocol for VMA21 antibody 21921-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency | ||
J Transl Med Hsa_circ_0001361 facilitates the progress of lung adenocarcinoma cells via targeting miR-525-5p/VMA21 axis. | ||
Autophagy Aerobic exercise attenuates autophagy-lysosomal flux deficits by ADRB2/β2-adrenergic receptor-mediated V-ATPase assembly factor VMA21 signaling in APP-PSEN1/PS1 mice |
